Method for quickly propagating breeding peanut seeds
A technology of original peanuts and peanuts, which is applied in the field of rapid propagation of original peanuts, can solve the problems of slow promotion of improved peanuts and low reproduction coefficient of original peanuts, and achieves the purpose of maintaining the characteristics of ancestors, accelerating the speed of promotion, and improving reproduction. The effect of the coefficient
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Embodiment 1
[0010] Use 1% HgCl to sterilize the surface of 1 original peanut seed of Huayu No. 30, disinfect it for 15 minutes, rinse it with sterile water for 3 times after disinfection, sow it in a flowerpot filled with sterilized nutrient soil, and put Place the flower pot in the tissue culture room; after the flower grows to 10 leaves, take 8 young peanut leaves, and disinfect the surface of the young peanut leaves with 0.02% to 0.03% HgCl for 15 minutes, rinse with sterile water after disinfection 3 times, cut the rinsed young peanut leaves into 8 leaflet slices of 1.5mm in length and width; put the young peanut leaf slices into the bud induction medium, and the formula of the bud induction medium used was MS+1mg / L 6-BA+ 0.5mg / L 2,4D; enter the elongation medium culture after the end of the shoot induction medium, the elongation medium formula used is MS+0.5mg / L 6-BA; use the rooting medium after the end of the elongation medium culture The culture medium is used for tissue culture, ...
Embodiment 2
[0012] Use 1% HgCl to sterilize the surface of 1 original peanut seed of Huayu 23, disinfect it for 15 minutes, rinse it with sterile water for 3 times, sow it in a flowerpot filled with sterilized nutrient soil, and place the flowerpot Place in tissue culture chamber. After the flower grows to 10 leaves, take 7 young peanut leaves, sterilize the surface of the young peanut leaves with 0.02%-0.03% HgCl, disinfect for 15 minutes, rinse 3 times with sterile water after disinfection, and rinse the young peanut leaves after rinsing. The leaves were cut into 10 leaflet slices of 1.5mm in length and width; the young peanut leaf slices were put into the bud induction medium, and the bud induction medium formula used was MS+1mg / L 6-BA+0.5mg / L 2,4D; After the end of the shoot induction medium, enter the elongation medium culture, the elongation medium formula used is MS+0.5mg / L 6-BA; after the end of the elongation medium culture, use the rooting medium for tissue culture, the rooting ...
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