Living body imaging method for monitoring temperature change in plant flower bud differentiation or flowering process

A technology for flower bud differentiation and plant monitoring, applied in the biological field, can solve the problems of unreported, lagging in vivo research, lack of temperature measurement, etc., and achieve the effect of simple post-processing, good imaging effect, and fast image imaging

Active Publication Date: 2013-06-05
BEIJING FORESTRY UNIVERSITY
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  • Abstract
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Problems solved by technology

Due to the lack of effective experimental instruments and technical means for the instantaneous capture of the thermogenic effect of flowering and the measurement of the temperature inside the plant during plant reproduction and development, most of the existing research is to obtain experimental data from chemically fixed "dead cells". This may be very different from the results obtained in in vivo measurements, and because the subtle heat generated by respiratory metabolism in plant reproduction and development cannot be captured in a timely and effective manner. The technology has not been reported, which has led to the relatively lagging behind in vivo research on reproductive and development mechanisms such as flowering mechanisms. So far, researchers have not yet made substantial progress in revealing the mechanism of flowering and heat generation in reproductive and development mechanisms.
[0004] Infrared technology and infrared imaging have been used in construction, factory inspection and other fields since they were used, but their application in the field of plant research has not been reported, especially in the experimental field of plant reproductive development and floral organ differentiation. Not yet reported

Method used

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  • Living body imaging method for monitoring temperature change in plant flower bud differentiation or flowering process
  • Living body imaging method for monitoring temperature change in plant flower bud differentiation or flowering process
  • Living body imaging method for monitoring temperature change in plant flower bud differentiation or flowering process

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Embodiment 1

[0045] Embodiment 1, monitoring the thermogenic effect of magnolia flower bud differentiation

[0046] Using the method of the present invention to mark and display the thermal imaging of the internal differentiation region of the flower bud of Magnolia in the flowering stage comprises the following steps:

[0047] 1. Temperature calibration for plant emissivity:

[0048] 1) Use an infrared non-contact thermal imager, adjust the emissivity to 0.95, and set the spectral band to 8μm-14μm. Spatial resolution (IFOV) 0.47mrad;

[0049] 2) Turn on infrared scanning to record temperature, capture high and low temperature instantaneously, thermal sensitivity: ≤0.07°C at 30°C. Detector data collection frequency: 50-60Hz;

[0050] 3) Before the measurement, correct the imaging temperature: take the temperature of the ice-water mixture as the benchmark, remove the lens cover of the thermal imager, point the lens at the target, and rotate the lens with your fingers, and place the ice-w...

Embodiment 2

[0066] Embodiment 2, monitoring the thermogenic effect of magnolia flowering

[0067] Using the method of the present invention to the dioecious live body real-time dynamic infrared imaging of Magnolia flower organ, comprises the following steps:

[0068] 1. Temperature calibration for plant emissivity:

[0069] 1) Using an infrared non-contact thermal imager, the adjusted emissivity is 0.95, and the spatial resolution (IFOV) is 0.47mrad;

[0070] 2) Turn on infrared scanning to record temperature, capture high and low temperature instantaneously, thermal sensitivity: ≤0.07°C at 30°C. Spectral band 8μm-14μm, detector data acquisition frequency: 50-60Hz;

[0071] 3) Before the measurement, correct the imaging temperature: take the temperature of the ice-water mixture as the benchmark, remove the lens cover of the thermal imager, point the lens at the target, and rotate the lens with your fingers, and place the ice-water mixture in the ice box under infrared heat. Perform tem...

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Abstract

The invention discloses a living body imaging method for monitoring a temperature change in a plant flower bud differentiation or flowering process, which comprises the following steps of: 1, acquiring a flower bud or flower organ image of a living body plant to be monitored by adopting an infrared non-contact thermal imaging system; and 2, obtaining temperatures of different sites of the flower bud or flower organ of the living body plant to be monitored according to the acquired image obtained in the step 1 so as to monitor the induced thermogenesis of the plant flower bud differentiation or flowering. Experiments prove that the method has the advantages of simpleness and rapidness in operation so that forward test analysis steps of sampling, separating and the like in plant observationare omitted, and good imaging effect so that living body observation can be carried out on thermogenesis of flower organs of the plant under the condition that outside environment interference such as rain, snow and the like is avoided and the temperature difference discrimination is 0.1DEG C.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an in vivo imaging method for monitoring temperature changes during plant flower bud differentiation or flowering. Background technique [0002] The mechanism of reproductive development is an important feature that distinguishes a species from other species, and it is also a concrete manifestation of the co-evolution of the original population adapting to the environment during the historical evolution process. As the sexual reproductive organs of flowering plants, the floral organs play a vital role in the process of plant reproduction and development. Therefore, in the process of reproductive development of flowering plants, from the differentiation of flower buds to the maturity of flower organs, The entire developmental process from germination and growth of mature pollen on stigma to double fertilization to seed maturity has become the key field of plant reproductive developmen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01J5/00
Inventor 王若涵张志翔印中华王建中刘翔宇牟淑琳
Owner BEIJING FORESTRY UNIVERSITY
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