Fishskin zymolyte preservative as well as preparation method and preservation method thereof
A technology of preservative and enzymatic hydrolyzate, which is applied in the direction of preserving meat/fish with coating protection layer, so as to save energy, slow down growth and reproduction, and maintain the effect of quality and flavor
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Embodiment 1
[0020] (1) Preparation of fish skin enzymatic hydrolyzate preservative: weigh 100 g of carp skin, add 7 times the volume of water, stir for 30 min, pour off the water, repeat this operation 3 times, add 7 times the volume of distilled water, and use 1mol / l of hydrochloric acid to adjust the pH of the solution to 2; add pepsin accounting for 4% of the mass of fish skin, and enzymolyze it in a water bath at 45°C for 3h; Filter the supernatant and adjust the pH to 7.0 to obtain the fish skin enzymatic hydrolyzate, adjust the protein concentration in the fish skin enzymatic hydrolyzate to 35 mg / ml, and add glycerol accounting for 5% of the volume of the fish skin enzymatic hydrolyzate as plasticizer agent, the resulting solution is fish skin enzymatic hydrolyzate preservative.
[0021] (2) The method of fish skin enzymatic hydrolyzate preservative to keep fish fresh: kill the fresh carp, remove the scales, gills and viscera, put it in ice water for cooling, clean it and drain it ...
Embodiment 2
[0024] (1) Preparation of fish skin enzymatic hydrolyzate preservative: weigh 100 g of carp skin, add 6 times the volume of water, stir for 25 minutes, pour off the water, repeat the operation 3 times, add 6 times the volume of distilled water, and use 1mol / 1 hydrochloric acid to adjust the pH of the solution to 2; add pepsin accounting for 3% of the mass of fish skin to enzymatically hydrolyze in a water bath at 45°C for 3.5 hours, and immediately place it in a water bath at 100°C to inactivate the enzyme for 10 minutes after the enzymolysis is completed; Filter the supernatant and adjust the pH to 7.0 to obtain the fish skin enzymatic hydrolyzate, adjust the protein concentration in the fish skin enzymatic hydrolyzate to 30 mg / ml, and add glycerol accounting for 4% of the volume of the fish skin enzymatic hydrolyzate as plasticizer agent, the resulting solution is fish skin enzymatic hydrolyzate preservative.
[0025] (2) Fish skin enzymatic hydrolyzate preservative method ...
Embodiment 3
[0028] (1) Preparation of fish skin enzymatic hydrolyzate preservative: weigh 100 g of carp skin, add 6 times the volume of water, stir for 20 minutes, pour off the water, repeat the operation 3 times, add 5 times the volume of distilled water, and use 1mol / 1 hydrochloric acid to adjust the pH of the solution to 2; add pepsin accounting for 3% of the mass of fish skin to enzymatically hydrolyze in a water bath at 43°C for 4 hours, and immediately put it in a water bath at 100°C to inactivate the enzyme for 10 minutes after the enzymolysis is completed; filter with suction Take the supernatant and adjust the pH to 7.0 to obtain the enzymatic hydrolyzate of fish skin, adjust the protein concentration in the enzymatic hydrolyzate of fish skin to 25mg / ml, and add glycerin accounting for 5% of the volume of the enzymatic fish skin hydrolyzate as a plasticizer , the resulting solution is fish skin enzymatic hydrolyzate preservative.
[0029] (2) The method of fish skin enzymatic hy...
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