Method for getting excised embryo by virtue of intergeneric distant hybridization between radish and kohlrabi

A technology of distant hybridization and kohlrabi, which is applied in horticultural methods, botanical equipment and methods, plant genetic improvement, etc., can solve the problem of no distant hybridization between the two, and achieve the effect of improving compatibility.

Inactive Publication Date: 2011-10-12
INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no report on the study of distant hybridization between the two

Method used

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  • Method for getting excised embryo by virtue of intergeneric distant hybridization between radish and kohlrabi
  • Method for getting excised embryo by virtue of intergeneric distant hybridization between radish and kohlrabi
  • Method for getting excised embryo by virtue of intergeneric distant hybridization between radish and kohlrabi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0022] 1 Materials and methods

[0023] 1.1 Test materials

[0024] The radish male sterile line DH303A was used as the female parent (2n=18), and kohlrabi was used as the male parent (2n=18). The experimental materials were commercial products purchased from the Vegetable Science and Technology Research Center of Hubei Academy of Agricultural Sciences.

[0025] 1.2 Test method

[0026] This experiment was carried out at the Vegetable Science and Technology Center of Hubei Academy of Agricultural Sciences.

[0027] 1.2.1 Seeding

[0028] The kohlrabi and radish seeds were sown in the experimental greenhouse, and they were subjected to conventional field management.

[0029] 1.2.2 Bagging and tagging

[0030] The radish and kohlrabi are bagged after bolting. If there are flowers that have opened before bagging, the flowers should be removed. Each individual plant of the male sterile line of radish is bagged separately and marked with a serial number. There are 10 individua...

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Abstract

The invention relates to a method for getting an excised embryo by virtue of intergeneric distant hybridization between radish and kohlrabi, and the method is characterized by comprising the following steps in sequence: (1) preparing materials; (2) seeding; (3) bagging and hanging out a plate; (4) pollinating; and (5) performing embryo rescue. By utilizing the method provided by the invention, the excised embryo formed by virtue of intergeneric distant hybridization between the radish and the kohlrabi can be successfully obtained, and a hybrid embryo seedling is further cultured, thus the method provided by the invention is conductive to getting breeding new resources.

Description

technical field [0001] The invention belongs to the field of plant tissue culture rapid propagation, and in particular relates to a method for obtaining isolated embryos by distant hybridization between radish and kohlrabi. Background technique [0002] Radish (Raphanus sativus L.) is one of the main vegetable crops cultivated in our country. It is native to my country and is cultivated in various places. There are many varieties, such as white-skinned radish, red-skinned radish, and green-skinned radish. The roots are edible, either fresh or raw, and the seeds contain 42% oil, which can be used to make soap or lubricating oil. Seeds, fresh roots, and leaves can be used as medicine, which can lower Qi and eliminate accumulation. Raw radish contains amylase, which can help digestion. Kohlrabi (Brassica juncea Coss.var.napiformmis Palet.Bols) is a variety of mustard. It is mustard for roots. It is a biennial herb of Brassicaceae and Brassica. Its product organs are fleshy r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H4/00
Inventor 梅时勇甘彩霞崔磊何云启黄来春王晴芳丁自立张兴中梅雪飞高翔
Owner INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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