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DNA segment and its application

A fragment and nucleotide sequence technology, applied to DNA fragments and their application fields, can solve the problems of blind time-consuming, labor-intensive, and large-scale, and achieve the effect of preventing gene drift.

Inactive Publication Date: 2012-11-28
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional breeding of sterile lines, restorer lines and maintainer lines requires a large number of cross-combination experiments, which is blind and time-consuming.

Method used

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  • DNA segment and its application
  • DNA segment and its application
  • DNA segment and its application

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0030] Example 1. Acquisition of rice pollen-specific expression promoter and its detection and analysis

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Abstract

The invention discloses a DNA segment and its application. In the invention, the nucleotide sequence of the DNA segment is one of the sequences as described in 1), 2), and 3): 1) a nucleotide sequence represented by SEQ ID No. 1 in a sequence table; 2) a nucleotide sequence capable of being hybridized with the nucleotide sequence in 1) under extremely strict conditions and having promoter activity; 3) a nucleotide sequence sharing a homology above 70% with the nucleotide sequence in 1) and having promoter activity. Experiments in the invention show that: the DNA segment in the invention serves as a promoter and can specifically drive downstream GUS genes to begin to express from the late stage of single nucleic pollen until the time when the pollen completely matures.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a DNA fragment and its application. Background technique [0002] my country is a country with a large population and agriculture, but with the increasing population, it is impossible to guarantee sufficient food production to meet social needs only by simple agricultural planting models. Biotechnology is the most important way for sustainable agriculture. Traditional crop breeding relies on hybrid breeding, and the current intelligent sterile molecular design breeding technology is also being continuously improved. However, whether it is traditional methods or modern technologies, the establishment of male sterile lines plays an important role in plant breeding. As an important male reproductive organ, the development of anther is divided into two parts, one is the morphogenesis of anther, and the other is the formation and development of pollen. Whether the anther is normal or not ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21A01H5/00
Inventor 孟征吴凤刘源杜晓秋闫硕
Owner INST OF BOTANY CHINESE ACAD OF SCI
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