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Method for improving yield of ethanol produced by sugar beet fermentation

A technology for ethanol and sugar beet, applied in the field of fermentation engineering, can solve the problems of long fermentation time, high residual sugar in fermentation liquid, low fermentation efficiency and the like

Inactive Publication Date: 2014-01-08
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The invention provides a method for increasing the yield of ethanol produced by beet fermentation to solve practical problems such as high residual sugar in fermentation liquid, long fermentation time and low fermentation efficiency in the process of producing ethanol by beet fermentation. The purpose is to increase the yield of raw material ethanol, reduce manufacturing cost

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  • Method for improving yield of ethanol produced by sugar beet fermentation
  • Method for improving yield of ethanol produced by sugar beet fermentation
  • Method for improving yield of ethanol produced by sugar beet fermentation

Examples

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Embodiment 1

[0083] The invention provides a method for improving the yield of ethanol produced by sugar beet fermentation, comprising the steps of:

[0084] I. Preparation of Medium

[0085] (1) Yeast culture medium preparation: the dimensions of the material proportion involved in this step are all g / L.

[0086] a. Preparation of Pachysolen tannophilus liquid medium: xylose 15, glucose 40, yeast powder 5, peptone, MgSO 4 0.2, CaCl 2 0.5, KH 2 PO 4 1.0, pH4.5;

[0087] b. Pachysolomyces tannophilus solid medium: xylose 15, glucose 40, yeast powder 5, peptone 3, MgSO 4 0.2, CaCl 2 0.5, KH 2 PO 4 1.0, agar 15, pH4.5;

[0088] c. Preparation of Saccharomyces cerevisiae liquid medium: glucose 40, yeast powder 5, peptone 3, MgSO 4 0.2, CaCl 2 0.5, KH 2 PO 4 1.0, pH4.5;

[0089] d. Preparation of Saccharomyces cerevisiae solid medium: glucose 40, yeast powder 5, peptone 3, MgSO 4 0.2, CaCl 2 0.5, KH 2 PO 4 1.0, agar 15, pH 4.5.

[0090] (2) Preparation of fermentation medium:...

Embodiment 2

[0111] The invention provides a method for improving the yield of ethanol produced by sugar beet fermentation, comprising the steps of:

[0112] I. Culture medium preparation

[0113] (1) Yeast culture medium preparation: the dimensions of the material proportion involved in this step are all g / L.

[0114] a. Preparation of Pachysolen tannophilus liquid medium: xylose 20, glucose 50, yeast powder 8, peptone 4, MgSO 4 0.3, CaCl 2 1.0, KH 2 PO 4 2.0, pH5.0;

[0115] b. Pachysolomyces tannophilus solid medium: xylose 20, glucose 50, yeast powder 8, peptone 4, MgSO 4 0.3, CaCl 2 1.0, KH 2 PO 4 2.0, agar 17, pH5.0;

[0116] c. Preparation of Saccharomyces cerevisiae liquid medium: glucose 50, yeast powder 8, peptone 4, MgSO 4 0.3, CaCl 2 1.0, KH 2 PO 4 2.0, pH5.0;

[0117] d. Preparation of Saccharomyces cerevisiae solid medium: glucose 50, yeast powder 8, peptone 4, MgSO 4 0.3, CaCl 2 1.0, KH 2 PO 4 2.0, agar 18, pH 5.0.

[0118] (2) Preparation of fermentation ...

Embodiment 3

[0140] The invention provides a method for improving the yield of ethanol produced by sugar beet fermentation, comprising the steps of:

[0141] I. Culture medium preparation

[0142] (1) Yeast culture medium preparation: the dimensions of the material proportion involved in this step are all g / L.

[0143] a. Preparation of Pachysolen tannophilus liquid medium: xylose 25, glucose 60, yeast powder 10, peptone 5, MgSO 4 0.4, CaCl 2 1.5, KH 2 PO 4 3.0, pH5.5;

[0144] b. Pachysolen tannophilus solid medium: xylose 25, glucose 60, yeast powder 10, peptone 5, MgSO 4 0.4, CaCl 2 1.5, KH 2 PO 4 3.0, agar 20, pH5.5;

[0145] c. Preparation of Saccharomyces cerevisiae liquid medium: glucose 60, yeast powder 10, peptone 5, MgSO 4 0.4, CaCl 2 1.5, KH 2 PO 4 3.0, pH5.5;

[0146] d. Preparation of Saccharomyces cerevisiae solid medium: glucose 60, yeast powder 10, peptone 5, MgSO 4 0.4, CaCl 2 1.5, KH 2 PO 4 3.0, agar 20, pH 5.5.

[0147] (2) Preparation of fermentation ...

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Abstract

The invention provides a method for improving yield of ethanol produced by sugar beet fermentation. Composite alcohol enzyme is coordinated with ultrasonic wave to treat a sugar beet raw material, so that cellulose and hemicellulose in the sugar beet raw material are decomposed to generate fermentable sugar; the colloid is decomposed, so that pipeline blockage is avoided; and the protein is decomposed into amino acid and dipeptide, so that a nitrogen source is provided for saccharomycetes, the load of amino acid anabolism of saccharomycetes cells is lightened, and more substrate is turned to fermentation for producing the ethanol. Pentose and hexose can be simultaneously utilized in the adopted mixed yeasts. The method solves the problems of low yield of the ethanol produced by sugar beet fermentation, high residual sugar of fermentation solution, long fermentation time and the like. By the method, the ethanol content of the fermentation solution is increased by 2.77 to 3.67 percent; and at the temperature of 20 DEG C, the ethanol volume concentration of the fermentation solution is 9.3 to 12.8 percent, the residual total sugar is 0.15 to 0.45 percent, and the ethanol yield of the raw material is 12.9 to 14.1 percent.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, in particular to a method for increasing the yield of ethanol produced by beet fermentation. technical background [0002] Sugar beet is a saline-alkali and drought-resistant sugar crop, and it is also an energy crop that can be grown on marginal land. The main chemical components in its roots are sucrose and reducing sugar. At present, sugar beets are mainly used for sugar production in China. Since the price of white sugar in China’s domestic market fluctuates greatly, about every two or three years, when the sugar price is low, the purchase price of sugar beets is also low, which seriously affects farmers’ enthusiasm for planting sugar beets. Sugar beet has high yield, high sugar, wide adaptability, and strong stress resistance. It can be directly used by yeast to convert ethanol, which saves two energy-consuming processes of starch gelatinization and saccharification than sta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12R1/645C12R1/865
CPCY02E50/17Y02E50/10
Inventor 李文王鑫孙恩博温暖
Owner JILIN ACAD OF AGRI SCI
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