Sequences of Saccharum officinarum sucrose phosphate synthase B (SofSPSB) gene and encoded protein thereof
A technology of phosphate synthase and protein coding, which is applied in the direction of enzymes, enzymes, biochemical equipment and methods, and can solve problems such as lack of research
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[0017] 1. Plant SPS (sucrose phosphate synthase) gene evolution analysis: use "sucrose phosphate synthase" to search the NCBI website (http: / / www.ncbi.nlm.nih.gov) non-redundant amino acid database to obtain all plant SPS sequences. All sequences obtained were analyzed to remove repetitive sequences. A total of 77 plant SPS sequences were obtained, including 43 full-length sequences. Align the amino acid sequences of all full-length SPS with ClustalX, and save the alignment results in PHYLIP format; then use the Seqboot, Protdist, Neighbor and Consense programs of the PHYLIP software package to perform evolutionary analysis, and divide all full-length SPS genes in plants for the corresponding family.
[0018] 2. Design primers for amplifying the core fragment of the SoSPSB gene: compare the cDNA sequences of the plant B family SPS gene, and design a pair of primers for amplifying the partial fragment of SofSPSB in the conserved region:
[0019] SofSPSB-F(5'-atgaacttcaacccctc...
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