Method for encoding microcarrier

An encoding method and a technology of microcarriers, which are applied in the application field of promoting microcarriers

Inactive Publication Date: 2011-08-10
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

More importantly, because the above coding methods often require expensive detection instruments to decode, it is difficult to reduce the cost, thus limiting the further popularization and application of microcarriers.
Moreover, the analysis method combined with flow cytometry is also difficult to compete with the analysis speed of the microarray chip.

Method used

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  • Method for encoding microcarrier
  • Method for encoding microcarrier
  • Method for encoding microcarrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] First, prepare polystyrene sheets with A, B, and C raised text characters with a surface character size of about 4 mm and a height of about 0.1 mm by injection molding, and prepare by cutting about 5 mm with A, B, and C characters. Then treat the upper surface of the wafer with nitrogen plasma gas to make the surface with amino groups, and then use the linking reagent glutaraldehyde to connect the hepatitis A antibody on the surface of the wafer engraved with the protruding "A" to obtain a sensor capable of detecting hepatitis A antigen. functional unit. Repeat the above steps to prepare disc-shaped polystyrene sensing functional units capable of detecting hepatitis B and hepatitis C with B and C character codes in batches. Put one piece of each of the above three sensing functional units into a microbox with a transparent top cover and a flat bottom of about 2 cm in diameter, and mix it with human serum samples to react, pour the serum samples out and wash them with PB...

Embodiment 2

[0026] First obtain nickel plates with different protruding text characters A1 (code A stream), A2 (code B stream), A3 (code C stream) (character size about 2 mm, depth about 0.1 mm), and then separate them by hot pressing Prepare polyethylene sheets with recessed characters A1, A2, A3 and mechanically cut them to obtain polyethylene discs with a diameter of about 3 mm and recessed characters. After ammonia plasma treatment, use glutaraldehyde to correspond to the text on the wafers, respectively connect one of the influenza B virus monoclonal antibodies, influenza A virus monoclonal antibodies and influenza C virus monoclonal antibodies in batches, and then take one piece each Put it into a microbox with a diameter of about 1 cm and inject the blood sample to be analyzed. After the reaction, add gold-labeled three influenza virus polyclonal antibody solutions, wash with PBS buffer and observe with the naked eye to see whether there is colloidal gold on the surface of the lette...

Embodiment 3

[0027] Embodiment 3: Firstly, the characters A, B, and C are printed on the transparencies by a printer, and then a square sheet with a size of about 2 mm is formed by mechanical cutting, and then the circular sheet is covered by plastic sealing technology and cut again by mechanical A disc with a size of about 2.5 mm is prepared. After treating the above-mentioned squares with ammonia plasma, use glutaraldehyde to correspond to the words of the squares to connect respectively in batches one of the monoclonal antibodies to influenza B virus, monoclonal antibodies to influenza A and monoclonal antibodies to influenza C, and then Take one piece each and put it into a microbox with a diameter of about 1 cm, inject the blood sample to be analyzed and add the solution of fluorescently labeled three influenza virus polyclonal antibodies after reaction, wash with PBS buffer and observe through a fluorescent microscope to pass through the disk The presence or absence of fluorescence o...

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Abstract

The invention provides a method for encoding a microcarrier and relates to a mode for encoding the microcarrier and application thereof, in particular to a method for promoting the application of the microcarrier by combining an identifiable character serving as a code with the microcarrier. The microcarrier is a movable sensing device applicable to multi-target detection and analysis. The microcarrier is needed to be encoded so as to be identified during detection and analysis. In the conventional microcarrier detection and analysis technology, expensive instruments are needed frequently to decode and analyze the microcarrier with a relatively low analysis speed; therefore, in the method, the word character which is easy to identify, manufacture and popularize and rich in expression is used for encoding the microcarrier, so that the use performance of the microcarrier is expected to be improved and the application range of the microcarrier is extended.

Description

technical field [0001] The invention relates to a coding mode of a microcarrier and its application, in particular combining easily identifiable characters as codes with the microcarrier so as to promote the application of the microcarrier. Background technique [0002] Multifunctional sensor devices capable of detecting multiple materials at the same time are the development direction of biological and chemical sensor devices due to their small size, large amount of information, good performance, convenient use, and high reliability. of high attention. At present, multifunctional sensor devices for biological and chemical materials are mainly concentrated on array sensor chips and microfluidic chips. The array sensor chip mainly prepares an array of sensing sensitive materials by spotting and in-situ synthesis on the surface of a certain carrier, and then reacts with the sample to detect various biological and chemical substances. The main problems of this method are the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06K19/06
Inventor 张继中
Owner SOUTHEAST UNIV
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