Method for analyzing algae and chormophoric dissolved organic matters on line by non-external sheath fluid flow cytometry

Abstract

The invention relates to a method for analyzing algae and chormophoric dissolved organic matters (CDOM) on line by non-external sheath fluid flow cytometry, which comprises the following steps of: collecting and treating samples, detecting the algae, filtering the algae, detecting CDOM and outputting a result and calculating. In the method, sheath fluid is non-external sheath fluid, namely after the samples are subjected to primary treatment and filtered to remove the algae, the sheath fluid can be used directly, so the trouble of replacing the sheath fluid is saved and the biological pollution of a liquid fluid system in a system is prevented. By the method, the algae and the chormophoric dissolved organic matters (CDOM) in water can be detected and analyzed on line simultaneously, and an early warning can be given for the eutrophic degree of water quality so as to prevent the breakout of cyanobacterial blooms. No matter a cyclic sheath fluid mode or an acyclic sheath fluid mode is adopted in the flow cytometry, the CDOM can be detected effectively, and the accuracy of detection results of the algae is improved; and more water quality information can be acquired under the condition of increasing limited use cost.

Description

technical field [0001] The invention belongs to the technical field of online detection and analysis of water quality components, and relates to the detection of algae and colored soluble organic matter (Chromophoric dissolved organic matter, CDOM) in water. Soluble organic matter method. Background technique [0002] Algae refers to a class of eukaryotes in the Protist kingdom (some are also prokaryotes, such as algae in the cyanobacteria). Mainly aquatic, without vascular bundles, capable of photosynthesis. It contains phycobiliprotein, whose main function is to serve as a light-harvesting pigment complex for photosynthesis, which is formed by covalently combining the pigment group phycobililin and the carrier protein. It is divided into four types: phycoerythrin, phycocyanin, phycoerythrin and allophycocyanin. Due to their different contents in different algae, some algae appear red, while others are green. The protein contained in domestic cyanobacteria is mainly phyc...

AI Technical Summary

Problems solved by technology

At present, this test can only be done on a fluorescence spectrophotometer. The professional equipment used in the test is not only expensive, but also bulky and cannot be moved. The test results are not continuous, and the online detection of colored soluble organic compounds cannot be realized.

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