Preparation method of bromelin

Abstract

The invention relates to a preparation method of bromelin, and the specific steps are as follows: 1) extracting the bromelin, adding an organic solvent into pineapple condensed juice after ultra-filtration by a PSA (pressure sensitive adhesive) film for precipitating an enzyme, and further performing drying under reduced pressure and other steps for getting a high-activity enzyme product; and 2) purifying the bromelin, (i) utilizing polyacrylic acid fixed by magnetic ferric oxide microspheres for adsorbing the bromelin, wherein the adsorption rate of the bromelin approaches 100%, the pH value is 6-8, 0.01-0.15mol/L of phosphoric acid buffer solution and KCl with the concentration of 0.6-2.0mol/L are adopted as eluent for performing elution, and the adsorption process and the desorption process are completed within 0.5-1.5 minutes; and (ii) using a double-water phase system of a copolymer of epoxy ethane and epoxypropane to separate and extract the high-purity bromelin. The preparation method has the advantages that the production is simple, and the large-scale industrial production can be performed; and the bromelin is derived from natural plants, thereby having the characteristics of safety, non-toxicity, wide application range and the like; and the application is wide.

Description

【Technical field】 [0001] The invention relates to the technical field of bromelain, in particular to a preparation method of bromelain. 【Background technique】 [0002] Bromelain is a general term for a class of proteolytic enzymes extracted from pineapple plants. It mainly exists in pineapple stems and fruits. According to different extraction parts, it can be divided into stem bromelain and fruit bromelain. As an active protein, bromelain is easily affected by temperature, pH, metal ions, etc. The optimum reaction temperature of bromelain is between (55-60) ℃, the higher the temperature, the heat inactivation of the enzyme will be enhanced, and the reaction speed will decrease; the optimum pH of bromelain is 6.5-7.5, reaching the maximum value around 7.1, and the In an acidic environment, the reaction speed of the enzyme decreases rapidly, and it has a wider application range in an alkaline environment. The pH range of the enzyme is more stable in the range of 3.5 to 4.5. ...

AI Technical Summary

Problems solved by technology

Compared with the previous two methods, this method has less operation steps, mild conditions, reduces the loss of enzyme activity, and requires less investment in production and environmental protection, but the obtained enzyme products contain more miscellaneous proteins without enzymatic activity. The molecular weight of such proteins is similar to that of bromelain , so the ultrafiltration process cannot remove