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Method for online separation and measurement of biomass concentration by optical fiber attenuation total-reflection sensor

A technique of attenuating total reflection and biomass, applied in the measurement of phase influence characteristics, etc., can solve the problem of expensive demodulation equipment

Inactive Publication Date: 2013-05-08
CHONGQING UNIV
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Problems solved by technology

The fiber Bragg grating method and the long-period fiber grating method have higher temperature measurement accuracy, but the demodulation equipment is more expensive

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  • Method for online separation and measurement of biomass concentration by optical fiber attenuation total-reflection sensor
  • Method for online separation and measurement of biomass concentration by optical fiber attenuation total-reflection sensor
  • Method for online separation and measurement of biomass concentration by optical fiber attenuation total-reflection sensor

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Embodiment Construction

[0056] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0057] A method for on-line separation and measurement of biomass concentration by an optical fiber attenuated total reflection sensor, the method comprises the following steps:

[0058] 1. Production of sensor probes

[0059] 1. Production of corroded tapered optical fiber

[0060] Select multimode fiber, for example, the fiber core diameter is 1.0mm, the fiber outer diameter is 2.2mm, the core refractive index is 1.49, the bending radius is 2mm, the numerical aperture (NA) is 0.5, and the working temperature is -50~70℃ . According to the geometric dimensions of the photobiological hydrogen production reactor (the photobiological hydrogen production reactor is generally 400mm long, 200mm wide, and 200mm high), the optical fiber is made into a tapered optical fiber by melting and drawing, and the length L of the tapered optical fibe...

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Abstract

The invention discloses a method for the online separation and measurement of biomass concentration by an optical fiber attenuation total-reflection sensor, which comprises the steps: at first, the tip of a corroded taper fiber core is ground to be semispherical and then coated with a gold film in order to be manufactured as a bi-pass evanescent field absorption optical fiber; then the taper fiber core is manufactured helically into a sensing arm of the sensor while a reference arm and a temperature compensation arm are manufactured; and finally, the online separation and measurement for biomass concentration and culture medium concentration in the process of biochemical reaction is implemented through the relation formula between a signal voltage output by the sensing arm and a biomass refractive index at 293K as well as the relation formula between the signal voltage output by the reference arm and a culture medium refractive index at 293K. The sensor provided by the invention, which adopts the optical attenuation total-reflection form, has a measurement process free from the impact from solution turbidity, can realize the separation and the measurement of biomass concentration in a reactor and simultaneously eradicate the influences of temperature and optical source fluctuations on signals to be tested just by being inserted into bacterial suspension, and has the advantagesof accuracy for detection, sensitivity for reaction, high repeatability and long service life.

Description

technical field [0001] The invention relates to a method for detecting biomass concentration, in particular to a method for on-line separation and measurement of biomass concentration by an optical fiber attenuation total reflection sensor. Background technique [0002] Compared with the traditional method of converting fossil fuels through chemical methods and electrolyzing water to produce hydrogen, photobiological hydrogen production has the advantages of environmental friendliness, low energy consumption, and sufficient and extensive sources of hydrogen production substrates. However, in the photobiological hydrogen production process, the biochemical reaction process is very complicated, and there are many factors that affect the hydrogen production efficiency, such as the type of microorganisms, biomass concentration, medium concentration, medium flow rate, light intensity, pH value, and reactor temperature. And other factors. Among them, biomass concentration, medium...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/41
Inventor 钟年丙朱恂吴睿王永忠廖强丁玉栋王宏李俊叶丁丁
Owner CHONGQING UNIV
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