Improved gas permeable cell culture device and method of use

一种培养装置、渗透性的技术，应用在气体可渗透性细胞培养装置领域，达到降低给料频率、高效细胞培养、降低的速率的效果

Active Publication Date: 2011-06-08
WILSON WOLF MFG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The co-pending '814 low-cost device has limited scalability in the horizontal direction

Method used

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  • Improved gas permeable cell culture device and method of use
  • Improved gas permeable cell culture device and method of use
  • Improved gas permeable cell culture device and method of use

Examples

Experimental program
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Embodiment Construction

[0044] Figure 1A A perspective view of one embodiment of the invention is shown. The exterior of the gas permeable device 10 includes a top 18 , side walls 20 , rear side walls 21 and a bottom 19 . A gas compartment 24 extends downwardly from the top 18 into the gas permeable device 10 . The gas compartment 24 is configured to allow ambient gas to come into contact with the wall 22 . Selected wall 22 and / or regions of wall 22 are gas permeable. In this embodiment, there is one opening to the gas compartment 24 and this opening is located on the top 18 . Gas transfer through selected portions of gas compartment 24 is allowed, thereby allowing improved gas exchange between the contents of gas permeable device 10 and ambient gas. In this way, the device can belong to standard CO 2 The incubator, while the gas compartment 24 facilitates the gas exchange of the culture present within the gas permeable device. In addition to the gas compartment, the outer walls of the gas perm...

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PUM

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Abstract

Gas permeable devices and methods are disclosed that provide more efficient cell culture.

Description

[0001] related application [0002] This application claims the benefit of US Provisional Application No. 61 / 078,966, filed July 8, 2008, which is hereby incorporated by reference in its entirety. [0003] government concern [0004] This invention was made as part of a National Institutes of Health Small Business Innovation Research Grant R43 GM077778-01 supported by the US government and is entitled "High Efficiency Gas Permeable Cell Culture Device". technical field [0005] The technical field of the invention relates to gas permeable cell culture devices and cell culture methods that allow for a more efficient cell culture process. Background of the invention [0006] Each of the applications, patents and papers cited in this application and in co-pending U.S. Application No. 10 / 961,814, U.S. Application No. 11 / 952,848, U.S. Application No. 11 / 952,856, and references cited in each of these applications and references, including the prosecution of each co-pending paten...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/24
CPCC12M23/34C12M23/40C12M23/24C12M23/08
Inventor 约翰·R·威尔逊
Owner WILSON WOLF MFG
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