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Technological process for preparing stevioside from stevia rebaudiana bertoni

A technology of steviol glycoside and stevia, which is applied to the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of large loss and high cost of stevioside, achieve safe preparation process, low extraction cost, and reduce production cost effect

Inactive Publication Date: 2011-05-18
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

Disclosed in Chinese patent CN1876669A is a method for preparing high-purity medicinal steviol glycosides, the process passes Al 2 o 3 Purified by column chromatography, concentrated and then purified by silica gel column chromatography to prepare high-purity medicinal steviol glycosides, but the Al 2 o 3 Column chromatography and silica gel column chromatography separate and purify stevioside, the loss of stevioside is bigger, and Al 2 o 3 And silica gel can not be reused, the cost is higher

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  • Technological process for preparing stevioside from stevia rebaudiana bertoni

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Embodiment 1

[0027] Weigh 100 g of dried stevia leaves, place them in a countercurrent extraction device, add 1000 mL of water for countercurrent extraction, control the temperature at 50 ° C, and conduct countercurrent extraction for 2 hours. Take by weighing 2 parts of pre-treated macroporous adsorbent resin, each part of 120g, and put them into two glass columns with an internal diameter of 3cm and a column height of 36cm (diameter-to-height ratio 1:12) by wet method, and the two columns are connected in series (column I, column II column). The extract obtained by countercurrent extraction flows through series resin columns (column I, column II) for adsorption in sequence, the flow rate is 30BV / h, and the temperature of the resin column is controlled at 40°C. After adsorption, the residual liquid enters the countercurrent extraction device again for the second countercurrent extraction , the extraction time is 2h, the temperature is 50°C, after the extraction is completed, the extract i...

Embodiment 2

[0029]Weigh 1 kg of dried stevia leaves, place in a countercurrent extraction device, add 15 L of water for countercurrent extraction, control the extraction temperature at 60° C., and conduct countercurrent extraction for 1.5 h. Take by weighing 2 parts of pre-treated macroporous adsorbent resins, each part of 0.9Kg, put into two glass columns with internal diameter 6cm, column height 66cm (diameter-to-height ratio 1: 11) respectively by wet method, and two columns are connected in series (I column , II column). The extract obtained by countercurrent extraction was sequentially flowed through series resin columns (column I, column II) for adsorption at a flow rate of 30BV / h, and the temperature of the resin column was controlled at 40°C. After the adsorption, the residual liquid enters the countercurrent extraction device again for the second countercurrent extraction. The extraction time is 1.5h and the temperature is 60°C. After the extraction is completed, the extract is p...

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Abstract

The invention discloses a technological process for preparing stevioside from stevia rebaudiana bertoni. The technological process comprises the following steps: carrying out countercurrent extraction on the dried stevia rebaudiana bertoni raw material with water, sequentially adsorbing the obtained extracting solution with macroporous absorption resin columns which are connected in series, allowing the residual liquid after absorption to enter a countercurrent extraction device for cyclic extraction, reabsorbing the obtained extracting solution with the resin columns which are connected in series once again, and completing the extraction separation closed cycle process; after the whole countercurrent extraction and absorption process is completed, eluting the resin columns with water for removing impurities, desorbing with stripping liquid, collecting the stripping liquid, concentrating and drying to obtain the crude extract of the stevioside; and refining the crude extract by a solvent method and decolorizing resin respectively to finally obtain the stevioside product. In the obtained product, the content of the stevioside compound is 70-100%, wherein, two components such as the stevioside and rebaudioside account for 70-90% of the whole stevioside compound, thus meeting the standards for a medicine, a healthcare product or food.

Description

technical field [0001] The invention relates to a process for preparing steviol glycosides from stevia rebaudiana. Background technique [0002] Stevia (Stevia rebaudiana Bertoni.) is a perennial herb of the Asteraceae Stevia genus. It has strong adaptability and is widely distributed in my country. It can be harvested 2-3 times a year in the south and once a year in the north. Steviol glycoside is a natural non-caloric high-intensity sweetener extracted from stevia leaves. The main components are stevioside (Stevioside), rebaudioside A, B, C, D, E, F (Rebaudioside A, B, C, D, E, F), dulcoside A (Dulcoside A) and steviolbioside (Steviolbioside), wherein stevioside and rebaudioside A are the main sweetness components, and its sweetness is more than 300 times that of sucrose. Stevioside does not participate in metabolism, does not accumulate, and is non-toxic in the body. Its safety has been recognized by international organizations such as FAO and WHO. In 2004, the World Hea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/24C07H1/08
Inventor 邸多隆黄新异陈振斌
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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