Bacillussubtilis for decomposing aflatoxin and active protein secreted by same
A technology of Bacillus subtilis and aflatoxin, applied in the direction of microorganism-based methods, medical raw materials derived from bacteria, application, etc., can solve the problem of simultaneous degradation of aflatoxin B1 by Bacillus subtilis, the degradation efficiency is not high, and has not yet been found Degradation and other problems, to achieve good probiotics and stress resistance, high detoxification activity, specific effects
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Embodiment 1
[0064] Example 1 The cultivation method of Bacillus subtilis of the present invention
[0065] Take Bacillus subtilis ANSB324 CGMCC No. 36091.5ml (the concentration of viable bacteria is 10 9 CFU / ml), inoculated in 80ml medium for shaking flask fermentation culture, fermentation temperature is 37℃, pH value is 7.0, rotation speed is 200r / min, and fermentation time is 24h.
[0066] Among them, the shake flask fermentation medium consists of the following components: tryptone 10g, yeast extract 2g, glucose 2g, beef extract 3g, sodium chloride 4g, disodium hydrogen phosphate 3g, magnesium sulfate heptahydrate 1.0g, manganese sulfate 1.0g, 1000mL distilled water, pH 7.2.
[0067] After fermentation, the fermentation broth is stored in a refrigerator at 4°C for later use.
Embodiment 2
[0068] Example 2 The cultivation method of Bacillus subtilis of the present invention
[0069] Take Bacillus subtilis ANSB324 CGMCC No. 36090.5ml (the concentration of viable bacteria is 10 9 CFU / ml), inoculated in 50ml medium for shaking flask fermentation culture, fermentation temperature is 30℃, pH value is 7.0, rotation speed is 180r / min, and fermentation time is 20h.
[0070] Among them, the shake flask fermentation medium consists of the following components: tryptone 8g, yeast extract 1.5g, glucose 1.5g, beef extract 2g, sodium chloride 3g, disodium hydrogen phosphate 2.5g, and magnesium sulfate heptahydrate 0.5g , 1.0g manganese sulfate, 800mL distilled water, pH 7.0.
[0071] After fermentation, the fermentation broth is stored in a refrigerator at 4°C for later use.
Embodiment 3
[0072] Example 3 The cultivation method of Bacillus subtilis of the present invention
[0073] Take Bacillus subtilis ANSB324 CGMCC No. 36092.5ml (the concentration of viable bacteria is 10 9 CFU / ml), inoculated in 100ml medium for shaking flask fermentation culture, fermentation temperature is 40℃, pH value is 7.4, rotation speed is 220r / min, and fermentation time is 30h.
[0074] Among them, the shake flask fermentation medium consists of the following components: tryptone 12g, yeast extract 3g, glucose 4g, beef extract 5g, sodium chloride 6g, disodium hydrogen phosphate 4g, magnesium sulfate heptahydrate 1.5g, manganese sulfate 1.5g, 1200mL distilled water, pH 7.4.
[0075] After fermentation, the fermentation broth is stored in a refrigerator at 4°C for later use.
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