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High-efficiency insecticidal fungus and applications thereof

A fungal and efficient technology, applied in the direction of pesticides, fungi, applications, etc., to achieve unique control effects, high-efficiency control effects, and environmentally friendly and compatible effects

Inactive Publication Date: 2012-05-30
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, different strains have strong specificity in the virulence of different pest species

Method used

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  • High-efficiency insecticidal fungus and applications thereof
  • High-efficiency insecticidal fungus and applications thereof
  • High-efficiency insecticidal fungus and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The isolation and purification of embodiment 1 Metarhizium anisopliae MBJQH2-2 bacterial strain

[0028] Soil and grub corpses infected with Metarhizium anisopliae were collected from farmland or forest land in Hebei, Shandong, Jiangsu, Beijing, Guizhou and other places, and the microorganisms were isolated and purified in the laboratory.

[0029]The separation and purification steps are as follows: carefully pick the bacteria from the insect corpses, inoculate them on the selective medium, cultivate them at 25°C for 7 days, distinguish the colonies and transfer them to a new selective medium plate, and inoculate them at 25°C. Cultivate at low temperature for more than 14 days. After the colony is completely sporulated, transfer it to a test tube of the strain culture medium. After culturing at 25°C for 14 days, store it in a refrigerator at 4°C.

[0030] Preserve the purified Metarhizium anisopliae strains in the PDAY test tube culture medium, and obtain 12 bacterial s...

Embodiment 2

[0031] Morphological and biological characteristics of embodiment 2 bacterial strains

[0032] Prepare PDAY medium, its components and preparation method are as follows: add 200g of fresh potatoes and about 1000mL of water to boil for 15min, filter through gauze and leave the liquid, add 20g of glucose, 5g of yeast extract powder, and 15g of agar, heat to completely melt the agar, and quantify to 1000mL, divided into Erlenmeyer flasks, placed in a high-pressure steam sterilizer at 121°C for 20min, and when cooled to about 60°C, pour it into a petri dish to make a plate for use.

[0033] Inoculate conidia on PDAY plate medium, culture at 25°C for 2 days, colonies can be seen with the naked eye, and the colony hyphae are white and fluffy, continue to culture for 3-4 days, it can be seen that the colonies expand and grow, and green spores are produced on the surface, and the color gradually changes from light green Turns dark olive green.

[0034] Drop a drop of sterilized PDAY ...

Embodiment 3

[0035] Ribosome ITS region nucleotide sequence characteristics of embodiment 3 bacterial strains

[0036] Prepare LM medium, its components and preparation method are as follows: weigh 20g of sucrose, 5g of peptone, add water to 1000mL, divide into Erlenmeyer flasks, place in a high-pressure steam sterilizer at 121°C for 20min, let cool to room temperature for later use.

[0037] Inoculate the conidia into the above-mentioned medium, the inoculum amount is 2-3×10 5 Spores / mL, cultured on a shaker at 25±1°C and 200rpm for 48h, filtered through filter paper, retained mycelia, squeezed dry for later use.

[0038] Genomic DNA was extracted by CTAB method. According to the routine DNA extraction operation, the genomic DNA is obtained, such as figure 2 shown.

[0039] ITS1-5.8S-ITS2 rDNA region primer 15'-GTTTCCGTAGGTGAACCTGC and primer 25'-ATATGCTTAAGTTCAGCGGGT were used for PCR amplification to obtain amplified DNA fragments, such as image 3 , the fragment was subjected to n...

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Abstract

The invention relates to the field of biological control, in particular to a high-efficiency insecticidal fungus and applications thereof. The invention provides a high-efficiency insecticidal fungus metarhizium strain MBJQH 2-2 which effectively prevents and control scaraboid underground pests, is friendly and compatible with the environment and has a preservation number of CGMCC (China General Microorganism Conservation Center) No. 4275. The metarhizium strain of the invention is rooted in the nature, returns to the nature, is friendly and compatible with the environment, pollution-free andresidue-free, has unique high-efficiency control action on the scaraboid underground pests, can be used as a pollution-free novel pesticide and used in an innoxious production process of underground pests, such as crops, fruit trees, forest, lawn, and the like. Proved by field prevention and control tests, the invention has favorable prevention effect and reaches the prevention effect on a plurality of important scaraboid larvae up to 60-70 percent.

Description

technical field [0001] The invention relates to the field of biological control, in particular to a high-efficiency insecticidal fungus and its application. Background technique [0002] Agricultural, forestry, horticultural and other plants, pastures, and lawns are often attacked by diseases and insect pests, among which scarab pests are very serious, and there is a lack of efficient control methods. There are many kinds of scarabs in my country, 1500 kinds, and they are widely distributed. The main damage is in the larval stage. Because the larvae live in the soil throughout their life, they are highly concealed and eat a lot. damage. In general years, 10-30% of crops are lost, and in some areas, 50-80% is seriously lost, or even no harvest. The long-term use of chemical pesticides for prevention and control has significantly increased their resistance to pesticides. It is difficult to achieve effective control of general pesticides, and sometimes high-toxic pesticides ha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
Inventor 农向群刘春琴张泽华王广君
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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