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P53 fusion protein and application thereof

A fusion protein and protein technology, applied in the P53 fusion protein and application fields, can solve problems such as not yet produced

Inactive Publication Date: 2011-03-23
PROTEOMTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this study has not yet yielded therapeutic-scale biologically active recombinant p53

Method used

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  • P53 fusion protein and application thereof
  • P53 fusion protein and application thereof
  • P53 fusion protein and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0119] Example 1: Production of tetrameric p53 fusion proteins in Escherichia coli and identification of the produced p53 fusion proteins Certainly

[0120] Candidate protein drugs based on p53 should be wild-type to reduce immunogenicity, tetramer to improve drug efficacy, and need to be able to enter the targeted cancer cells. Prior to this invention, all attempts to produce wild-type, full-length, tetrameric p53 without chemical cross-modification were unsuccessful. As far as protein therapeutics are concerned, there are several reasons for isolating stable p53 tetramers. First, the active form of p53 is a tetramer. The p53 monomer binds DNA in a cooperative manner, and the binding force of p53 to DNA increases up to 100-fold after forming a tetramer (McLure, KG and Lee, PW 1998 EMBO J 17: 3342-3350). Second, tetramerization is also important for protein-protein interactions, and tetramerization regulates the binding of some proteins to p53. The tetramer structure al...

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Abstract

The invention relates to a p53 fusion protein and an application thereof. The invention provides the p53 protein and the p53 fusion protein in the tetrameric shape having the bioactivity. In the invention, the p53 protein and the p53 fusion protein inclusion body is produced by using the recombination expression by escherichia coli, and the inactivated p53 protein and the p53 fusion protein in the tetrameric shape and having the bioactivity are prepared by the renaturation and other steps. The invention also provides the medical application of the p53 protein and the p53 fusion protein.

Description

technical field [0001] The present invention relates to a composition containing recombinant tetrameric p53 protein and p53 fusion protein and a method for producing recombinant tetrameric p53 protein and p53 fusion protein in bacteria. The main application of the present invention relates to the field of medicine, in particular to a preparation medicine using tumor suppressor protein p53 fusion protein as anticancer protein medicine. Background of the invention [0002] TP53 (TRP53, p53) was first discovered more than 20 years ago. Because p53 is a well-characterized tumor suppressor protein with well-defined functions in DNA repair, cell proliferation, apoptosis and cancer, it is called "the guardian of the genome". p53 is a transcription factor that exists in the cytoplasm, and enters the nucleus after being activated by post-transcriptional modification, where it forms a tetramer and binds to DNA, activating genes that play an important role in cell regulation and DNA r...

Claims

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Application Information

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IPC IPC(8): C07K19/00A61K38/17A61K47/48A61P35/00C07K14/435A61K47/42
Inventor 蔺新力
Owner PROTEOMTECH
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