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Estimating method of genetic similarity index of codominance molecular marker based on relative correlation coefficient

A molecular marker and correlation coefficient technology, applied in the field of molecular biology, can solve the problem that the degree of kinship is not necessarily related

Active Publication Date: 2012-07-04
YUNNAN ACAD OF TOBACCO AGRI SCI +1
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Problems solved by technology

[0007] The purpose of the present invention is to overcome the defect that the existing genetic similarity index has no necessary connection with the degree of kinship between individuals, and to provide a method for estimating the genetic similarity index of co-dominant molecular markers based on the kinship correlation coefficient

Method used

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  • Estimating method of genetic similarity index of codominance molecular marker based on relative correlation coefficient
  • Estimating method of genetic similarity index of codominance molecular marker based on relative correlation coefficient
  • Estimating method of genetic similarity index of codominance molecular marker based on relative correlation coefficient

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Embodiment

[0032] The present invention is generally applicable to co-dominant molecular markers in the analysis and research of genetic diversity, taking the application of SSR molecular markers in the analysis of genetic diversity of rapeseed varieties as an example.

[0033] 1) Apply 8 pairs of polymorphic SSR primers to analyze 15 rapeseed varieties (Table 1), and obtain the electrophoretic patterns of 15 rapeseed varieties ( figure 1 ), according to the presence or absence of the amplified product band, read and record with 1 or 0 respectively, obtain the SSR fingerprint data (Table 2) comprising 22 amplified fragments;

[0034] Table 1 The 15 rapeseed varieties selected in the experiment

[0035]

[0036] Table 2 SSR fingerprint data of 15 rape varieties

[0037]

[0038] 2) According to the SSR fingerprint data of 15 rapeseed varieties, a pairwise comparison was carried out to obtain a, b, and c values ​​respectively. Taking Zheshuang 758 and Huayouza No. 4 rape varieties ...

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Abstract

The invention discloses a calculation method of genetic similarity index of codominance molecular marker based on relative correlation coefficient. The technical scheme is as follows: obtaining an electrophoresis pattern of each variety or kind of plant by adopting the current codominance molecular marker method; comparing in pairs according to the amplification strip information of the variety or kind of the plant; respectively calculating a genetic similarity index between each two plant varieties and kinds according to a calculation formula of the genetic similarity index; and taking the genetic similarity index as the estimation of the corresponding relative extent of the individuals. The calculation method of genetic similarity index is applied to the codominance molecular marker. The calculation method of the obtained genetic similarity index can effectively respond the relative relationship extent among the colonies based on the relative correlation coefficient.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a method for estimating genetic similarity index of co-dominant molecular markers based on kinship correlation coefficient. Background technique [0002] With the development of biotechnology, the detection of genetic diversity has gradually developed from the morphological, physiological and biochemical levels to the chromosome and DNA levels, and has experienced a development process from simple to complex, from macro to micro, and from qualitative to quantitative. [0003] The detection of genetic diversity at the DNA level is mainly through RFLP (restriction fragment length polymorphism), RAPD (random amplified polymorphic DNA), SSR (microsatellite polymorphism) and other techniques to analyze the nuclei of some specific genes or DNA fragments. nucleotide sequence, measure its variability, and detect a batch of recognition sites in the genome, thereby estimating the variabili...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G06F19/00C12Q1/68
Inventor 李永平胡晋黄歆贤马文广郑昀晔关亚静祝水金
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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