Freezing storage protective solution for human umbilical Wharton's jelly tissue block

A technology of Wharton's jelly tissue and human umbilical cord, which is applied in the field of cryopreservation protection solution, can solve the problems of cryopreservation protection solution residue, high toxicity and side effects, and achieve the effect of reducing residue, small side effects and reducing toxicity

Inactive Publication Date: 2011-02-16
江苏省北科生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under normal circumstances, people choose a single permeable cryoprotectant dimethyl sulfoxide to cryopreserve stem cells.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0011] Take 500ml of culture medium DMEM / F12, add 0.1mol sucrose and mix well, filter it with a 0.22um membrane filter, pre-cool at 4°C, add 100ml of autologous cord blood serum pre-cooled at 4°C on an ice bath, and pre-cool at 4°C 0.8 mol of ethylene glycol, 0.4 mol of dimethyl sulfoxide pre-cooled at 4°C, and supplemented with culture medium DMEM / F12 to 1 L is the cryopreservation protection solution for human umbilical cord Wharton's jelly tissue blocks.

example 2

[0013] Take 500ml of culture medium DMEM / F12, add 0.3mol of sucrose and mix thoroughly, filter it with a filter of 0.22um filter membrane, pre-cool at 4°C, add 200ml of autologous cord blood serum pre-cooled at 4°C on the ice bath, and pre-cool at 4°C. 1.2 mol of cold ethylene glycol, 0.6 mol of dimethyl sulfoxide pre-cooled at 4°C, and supplemented with culture medium DMEM / F12 to 1 L is the cryopreservation protection solution for human umbilical cord Wharton's jelly tissue blocks.

example 3

[0015] Take 500ml of culture medium DMEM / F12, add 0.2mol of sucrose and mix thoroughly, filter it with a filter of 0.22um filter membrane, pre-cool at 4°C, add 150ml of autologous cord blood serum pre-cooled at 4°C on the ice bath, and pre-cool at 4°C. 1 mol of cold ethylene glycol, 0.5 mol of dimethyl sulfoxide pre-cooled at 4°C, and supplemented with DMEM / F12 culture medium to 1 L is the cryopreservation protection solution for human umbilical cord Wharton's jelly tissue blocks.

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PUM

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Abstract

The invention discloses freezing storage protective solution for a human umbilical Wharton's jelly tissue block. 1 liter of the freezing storage protective solution comprises 0.8 to 1.2 mol of glycol, 0.4 to 0.6 mol of dimethyl sulfoxide, 0.1 to 0.3 mol of cane sugar, 100 to 200 milliliters of cord blood autoserum and the balance of culture solution DMEM/F12. In the freezing storage protective solution, the cord blood autoserum and the culture solution DMEM/F12 serve as base solution to provide nutrient for the tissue block, so osmotic equilibrium inside and outside cells is guaranteed, the cell environment in the tissue block is guaranteed to be similar to the in vivo environment, and the freezing storage effect is good; the low-concentration glycol and dimethyl sulfoxide serve as a permeability freezing protective agent, so the toxicity of the freezing storage protective solution to the cells is reduced; and the cane sugar serves as a non-permeability freezing protective agent, so the viscosity of the solution is increased and the osmotic pressure inside and outside cell membranes is relieved when the cells are frozen and stored. The human umbilical Wharton's jelly tissue block can be directly frozen and stored by the freezing storage protective solution, so the freezing storage effect is good, the cell damage is little, and high-quality human umbilical mesenchymal stem cells can be provided for clinic.

Description

technical field [0001] The invention relates to a cryopreservation protection solution for human umbilical cord Wharton's jelly tissue blocks. Background technique [0002] The cells in human umbilical cord Wharton's Jelly have the characteristics of stem cells such as self-renewal, multi-directional differentiation, and strong proliferation ability. We call them umbilical cord mesenchymal stem cells (huMSCs). Mesenchymal stem cells are an important member of the stem cell family. They are low-immunogenic stem cells. They have the characteristics of supporting hematopoiesis, promoting stem cell implantation, and regulating immunity. They are ideal seeds for repairing tissue and organ damage caused by aging and disease. cell. Umbilical cord mesenchymal stem cells are getting more and more attention because of their wide source, easy access, and all the characteristics of mesenchymal stem cells. More and more people start to store umbilical cord mesenchymal stem cells for fut...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 李伟杨兵欧阳溪宋云庆胡祥
Owner 江苏省北科生物科技有限公司
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