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Pathogenic aeromonas hydrophila assay kit

A technology of Aeromonas hydrophila and a detection kit, which is applied in the field of detection of pathogenic Aeromonas hydrophila, and can solve the problems of difficult analysis, few nucleotides, no genetic information classification research, etc.

Inactive Publication Date: 2011-01-12
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although 5S rRNA is easy to analyze, due to the lack of nucleotides, there is not enough genetic information for taxonomic research
The number of nucleotides contained in 23S rRNA is almost twice that of 16S rRNA, so it is more difficult to analyze

Method used

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  • Pathogenic aeromonas hydrophila assay kit
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  • Pathogenic aeromonas hydrophila assay kit

Examples

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Embodiment Construction

[0045] Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood by those skilled in the art that these examples are only used to illustrate the present invention and do not constitute any limitation to the scope of the present invention. Unless otherwise specified, all scientific and technical terms in this application have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Any patents, patent applications and publications cited in this application are hereby incorporated by reference. The experimental methods not indicating specific conditions in the following examples generally adopt conventional conditions, for example, the conditions described in "Molecular Cloning Laboratory Manual" (New York: Cold Spring Harbor Laboratory Press, 1989) (Sambrook et al.), or as recommended by the manufacturer.

[0046] (1) Preparation of pathogenic Aeromonas hydrophila detect...

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Abstract

The invention relates to a pathogenic aeromonas hydrophila assay kit and belongs to the field of the detection of pathogenic microorganisms. A primer which is designed on the basis of a highly conserved interval of a standard strain J-1 strain 16S rDNA genome of the pathogenic aeromonas hydrophila is used for distinguishing and identifying strain characteristics of the pathogenic aeromonas hydrophila, and the amplified fragment is 685bp; and a second pair of primers which is designed according to specific proteinase aerolysin secreted by the strain is used for judging the pathogenicity of the aeromonas, and the fragment size is 301bp. The assay kit can rapidly and accurately judge the strain characteristics and pathogenicity of the strains to be tested. Comparison tests of the conventional biochemical identification method and pathogenicity identification method for experiment strains and clinical stains show that the coincidence rate of the kit is 100 percent. Evaluation tests for specificity, repeatability, sensitivity and retention period of the kit acquire ideal results.

Description

technical field [0001] The invention relates to a pathogenic Aeromonas hydrophila detection kit, which belongs to the field of pathogenic microorganism detection and relates to the detection of pathogenic Aeromonas hydrophila for humans and susceptible animals. Background technique [0002] Aeromonas has a wide range of pathogenicity. It infects a variety of animals including cold-blooded animals such as fish, poultry and mammals, causing local infections such as sepsis or skin ulcers. It is the most common pathogenic bacteria in aquatic animals, especially fish. Germs, Aeromonas mobilis can cause outbreaks of fish bacterial sepsis in summer when the water temperature is high. Human infection with Aeromonas mobilis causes acute gastroenteritis, etc. At present, this bacteria has been included in the routine detection range of diarrhea pathogens in foreign countries, and it is the object of food hygiene inspection. Aeromonas hydrophila has also been found in human cases of s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04
Inventor 姚火春陆承平潘子豪
Owner NANJING AGRICULTURAL UNIVERSITY
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