Method for breeding cross-bred 'Hulongban' fish
A technology of tiger dragon and brown-spotted grouper, which is applied in application, climate change adaptation, fish farming, etc., to achieve the effects of simple operation, easy to master the operation method and high survival rate
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[0016] The specific method steps of hybridization "tiger dragon spot" breeding test are as follows:
[0017] (1) Breeding and screening of broodstock: The broodstock is usually raised in the broodstock breeding pond, fed with fresh miscellaneous fish, intensively cultivated one month before the breeding season, and fed with enhanced feed for the broodstock. Before mating, the broodstock were caught, and their traits and gonads were checked one by one. Choose healthy broodstock with no injuries or deformities on the body surface; female brown-spotted grouper requires obvious abdominal swelling and ovary development to stage IV; male saddleband grouper requires light pressure on the abdomen to have white semen flow out;
[0018] (2) Hybrid parent matching: select the individual brown-spotted grouper female as the female parent, and the saddle-banded grouper male as the male parent during mating;
[0019] (3) Oxytocin: During the grouper breeding season, inject oxytocin hormone ...
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