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Bacillus subtilis B2 microbial agent, compound microbial agent and application

A compound microbial inoculant, Bacillus subtilis technology, applied in the directions of microorganisms, biocides, microorganism-based methods, etc., can solve the problem of chemical pesticides with poor environmental protection, easy to produce drug resistance and poor safety, easy to produce drug resistance and safety, etc. question

Active Publication Date: 2010-12-29
陈秀蓉 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The first purpose of the present invention is to provide a strain of Bacillus subtilis to utilize the broad-spectrum bactericidal activity and promoting activity of chemical pesticides in the prior art, which are poor in environmental protection, easy to produce drug resistance and poor in safety. production ability, the preparation of environmental protection, not easy to produce drug resistance and good safety of Bacillus subtilis B2 microbial inoculum
[0016] The second purpose of the present invention is to provide a kind of bacillus subtilis B2 microbial inoculum for the disadvantages of poor environmental protection, easy to produce drug resistance and poor safety of chemical pesticides in the prior art, so as to realize good environmental protection and difficult to produce drug resistance of microbial pesticides and good security

Method used

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  • Bacillus subtilis B2 microbial agent, compound microbial agent and application
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  • Bacillus subtilis B2 microbial agent, compound microbial agent and application

Examples

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Embodiment 1

[0107] According to an embodiment of the present invention, a kind of bacillus subtilis B2 microbial inoculum is provided, and this microbial inoculum is fermented in bovine peptone medium by the inoculum of 5% bacillus subtilis B2, when the active bacillus subtilis B2 The number of bacteria reaches 1.007×10 10 cfu / ml, the obtained fermented liquid can be directly used as the Bacillus subtilis B2 microbial inoculum, and the pH value of the fermented liquid can reach 7.5.

[0108] Among them, the beef peptone medium includes 10 g of glucose, 6 g of beef peptone and 3.5 g of yeast extract, and the volume is adjusted to 1000 ml with distilled water or deionized water. Here, the pH of beef peptone medium can reach 7.0.

[0109] During the liquid fermentation of the above-mentioned Bacillus subtilis B2 in the beef peptone medium, many foams are unavoidably produced, and rapeseed oil accounting for 0.8% by weight of the beef peptone medium can be added as an antifoaming agent to el...

Embodiment 2

[0112]In the present embodiment, a kind of Bacillus subtilis B2 microbial inoculum is provided, and this microbial inoculum is fermented in beef peptone medium by the Bacillus subtilis B2 that the inoculum amount is 7.5%, when the active Bacillus subtilis B2 The number of bacteria reaches 1.50×10 11 When the cfu / ml is used, the fermented liquid obtained can be directly used as the Bacillus subtilis B2 microbial bacterial agent, and the pH value of the fermented liquid can reach 7.8.

[0113] Among them, the beef peptone medium includes 15 g of glucose, 8 g of beef peptone and 5.5 g of yeast extract, and the volume is adjusted to 1000 ml with distilled water or deionized water. Here, the pH of beef peptone medium can reach 7.5.

[0114] During the liquid fermentation of the above-mentioned Bacillus subtilis B2 in the beef peptone medium, many foams are unavoidable, and rapeseed oil with a weight of 1.0% of the beef peptone medium can be added as an antifoaming agent to elimina...

Embodiment 3

[0117] In the present embodiment, a kind of bacillus subtilis B2 microbial inoculum is provided, and this microbial inoculum is fermented in beef peptone medium by the inoculum of 10% bacillus subtilis B2, when the live bacillus subtilis B2 The number of bacteria reaches 1.73×10 12 cfu / ml, the fermented liquid obtained can be directly used as the Bacillus subtilis B2 microbial inoculum, and the pH value of the fermented liquid can reach 8.0.

[0118] Among them, the beef peptone medium includes 20 g of glucose, 10 g of beef peptone and 6.5 g of yeast extract, and the volume is adjusted to 1000 ml with distilled water or deionized water. Here, the pH of beef peptone medium can reach 8.0.

[0119] During the liquid fermentation of the above-mentioned Bacillus subtilis B2 in the beef peptone medium, many foams are unavoidably produced, and rapeseed oil whose weight accounts for 1.2% of the beef peptone medium can be added as a defoamer to eliminate the foam produced during the f...

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Abstract

The invention discloses a bacillus subtilis B2 microbial agent, a compound microbial agent and an application thereof, wherein the microbial agent is obtained through liquid fermentation of bacillus subtilis B2 in a beef peptone culture medium, and the living bacteria number is 1.007*1010-1.73*1012cfu / ml; the compound microbial agent comprises the bacillus subtilis B2 and at least one strain of bacillus sp B3, bacillus sp B4, bacillus sp B5 and bacillus sp B6; the total living bacteria number is 1.33*109-4.06*1010cfu / ml; and the microbial agent and the compound microbial agent can inhibit pathogenic bacteria such as sclerotium bacterial of eggplants, botrytis cinerea of tomatoes and the like. The microbial agents and the application can overcome the defects of having poor environmental protection and poor safety, being easy to produce drug resistance and the like of the chemical pesticide in the prior art so as to realize the advantages of having good environmental protection and good safety and being difficult to produce the drug resistance.

Description

technical field [0001] The present invention discovers a strain of Bacillus subtilis for the first time. The Latin name of the strain is Bacillus subtilis B2, and the preservation number of the strain in the General Microorganism Center of China Microorganism Strain Collection Management Committee is CGMCC No.1747. [0002] The invention relates to the field of microbial pesticides, in particular to a preparation method and application of a Bacillus subtilis B2 microbial agent and a composite microbial agent. Background technique [0003] In order to protect the ecological environment, the "World Conference on Environment and Development" in 1992 proposed that the use of chemical pesticides should be controlled and gradually reduced on a global scale, and the use of biological pesticides should be increased. Our government also attaches great importance to this work and has introduced "green food and bio-pesticide development" into the 21st century agenda. [0004] As we al...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07C12R1/125
Inventor 陈秀蓉杨成德薛莉刘晓妹盛红梅王艳辛中尧闫沛迎李美丽荆卓琼
Owner 陈秀蓉
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