Preparation process for Sappan Wood extract perfusate and application thereof in treating bladder cancer
A preparation process and perfusate technology, applied in the directions of drug delivery, pharmaceutical formulation, etc., can solve the problem of not finding the research report of hematoxylin extract on bladder cancer, etc., and achieve the effect of good development prospect, good therapeutic effect and remarkable curative effect.
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Embodiment 1
[0082] Embodiment 1, a preparation process of bladder perfusion solution, the steps include: take hematoxylin and pulverize it, add 5 times the weight of purified water for the first time, boil and extract for 60 minutes to filter to obtain the filtrate, then add 3 times the weight of purified water for the second time, boil Extract for 40 minutes and filter to obtain the filtrate, then add 2 times the weight of purified water for the third time, boil and extract for 30 minutes and combine the extracts obtained three times, concentrate under reduced pressure to 2 times the weight of the crude drug; let stand overnight to remove the precipitate; then add a Double the volume of petroleum ether, extract, and keep the water phase; add three times the volume of ethyl acetate, extract, remove the water phase; evaporate the ethyl acetate under reduced pressure; weigh the dry matter, add purified water, and make the dry matter The content is 2%, heating and dissolving. Let it stand at...
Embodiment 2
[0095] Embodiment 2, a preparation process of bladder perfusion solution, the steps include: taking hematoxylin and pulverizing it, adding 3 times the amount of purified water for the first time, boiling and extracting for 65 minutes and filtering to obtain the filtrate, then adding 2 times the amount of purified water for the second time, boiling Extract for 50 minutes and filter to obtain the filtrate, then add 1.5 times the amount of purified water for the third time, boil and extract for 35 minutes; combine the extracts obtained three times, concentrate under reduced pressure to 2 times the crude drug amount of Sappanol; let stand overnight to remove the precipitate; Then add twice the amount of petroleum ether, extract, and keep the water phase; add four times more ethyl acetate, extract, and remove the water phase; evaporate the ethyl acetate under reduced pressure; weigh the dry matter, add purified water, and make the dry matter content 2%, heat to dissolve, stand at ro...
Embodiment 3
[0096] Embodiment 3, a preparation process of bladder perfusion solution, the steps include: taking hematoxylin and pulverizing it, adding 6 times the amount of purified water for the first time, boiling and extracting for 45 minutes to filter to obtain the filtrate, then adding 4 times the amount of purified water for the second time, boiling Extract for 30 minutes and filter to obtain the filtrate, then add 2.5 times the amount of purified water for the third time, boil and extract for 25 minutes; combine the extracts obtained three times, concentrate under reduced pressure to 2 times the crude drug amount of Sappan; let stand overnight to remove the precipitate; Then add 1.5 times the amount of petroleum ether, extract, and keep the water phase; then add 2 times the amount of ethyl acetate, extract, and remove the water phase; evaporate the ethyl acetate under reduced pressure; weigh the dry matter, add purified water, and make the dry matter The content is 2%, dissolved by ...
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