Method for breeding a large number of microplitis bicoloratus
A technology of ditch wasp and side ditch, which is applied in the field of insect management, can solve the problems of artificial mass reproduction of double-spot ditch wasp, achieves the effects of improving labor productivity and equipment use efficiency, preventing germplasm decline, and saving costs
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[0021] 1. Host breeding: use beet armyworm or Spodoptera litura larvae from the end of the 2nd to the beginning of the 3rd instar as the intermediate host, including host egg collection, egg hatching, larva rearing, and adult feeding;
[0022] The host eggs were hatched at a constant temperature of 25°C. After hatching, the larvae were raised in plastic square boxes (4cm high, 16cm long, and 16cm wide) filled with artificial feed. The breeding density was 200 worms / box, and the feeding conditions: 25-27 °C, RH=60-70%, photoperiod L:D=12:12, light intensity 500LUX. It can be provided to parasitic wasps for parasitism at the end of the 2nd age to the beginning of the 3rd age.
[0023] Host adults are placed in sarongs, fed with 10% honey water, and allowed to lay eggs on the ovum-laying paper.
[0024] 2. Receiving bees: according to the ratio of parasitic bees: host number of 1:5-10, use self-made plexiglass cages for inoculation. The plexiglass cages are 28cm long, 28cm wide,...
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