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Primers for IMP molecular markers of tobacco

A molecular marker, tobacco technology, applied in the fields of biotechnology and genetic engineering

Inactive Publication Date: 2010-12-01
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports of application in tobacco
At the same time, the search for MITEs in the whole genome of tobacco and the development of molecular markers using them have not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0010] In the present invention, the completion steps of the tobacco IMP molecular marker primer are as follows:

[0011] Step 1: Acquisition of Tobacco Genome Data

[0012] De-redundancy, splicing and sorting the original sequences released by the American Tobacco Genome Sequencing Project to obtain the original data for searching MITE;

[0013] Step 2: Tobacco Candidate MITE Lookup

[0014] According to the structural characteristics of MITE: terminal inverted repeat (TIR) ​​and target site repeat (TSD), use bioinformatics methods to obtain tobacco candidate MITE sequences;

[0015] Step 3: MITE-based primer selection

[0016] Based on the candidate MITE sequences obtained in the previous step, divide the MITE sequences with the same TIR structure into a subset, and perform multiple sequence alignment on the sequences in this subset to find the conserved domains in the MITE structure, and use this as the core to select 18 Primers with a length of ~20 nucleotides, such as ...

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PUM

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Abstract

The invention relates to the field of plant genetic engineering, and aims to provide primers for IMP molecular markers of tobacco. The primers have nucleotide sequences shown as SEQ ID NO:1 to SEQ ID NO:10. The invention belongs to the technology of new biomolecular markers of the tobacco. The provided primers can be used for the IMP molecular markers of the tobacco. The fields for the IMP molecular markers mainly comprise (1) auxiliary selection breeding of the molecular markers of the tobacco, namely the selection breeding for tobacco varieties; (2) the construction of a genetic map of the tobacco; (3) the positioning and discovery of important functional genes of the tobacco; (4) the early diagnosis and screening of specific properties of the tobacco; and (5) the judgment of the genetic relationship of the tobacco (such as the mixing of the tobacco varieties, the analysis of the tobacco and wild species thereof and the like).

Description

technical field [0001] The invention belongs to the field of biotechnology and genetic engineering, and relates to a molecular marker technology based on a miniature inverted repeat transposable element (Miniature inverted-repeat transposable element). Specifically, it is a primer for molecular marker of tobacco IMP. Background technique [0002] Common tobacco (Nicotiana tabacum) belongs to the Solanaceae (Solanaceae) Nicotiana plant, and is an important economic crop. Although tobacco plays an important role as a transgenic model plant in the field of biotechnology and genetic engineering, its own genetic research is relatively backward, and a high-density genetic map covering the entire genome has not yet been built, which severely limits the understanding of important agronomic traits of tobacco. The study of the genetic basis and laws of heredity. In view of the fact that tobacco has many chromosomes, large genome, and narrow genetic background, conventional molecular...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 樊龙江肖炳光桂毅杰童治军
Owner ZHEJIANG UNIV
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