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Tissue culture rapid propagation method of populus tremula multiplied by P. tremuloides

A technology of tissue culture and aspen, which is applied in the field of tissue culture and rapid propagation of European and American aspen hybrids. Long emergence time and other problems, to achieve the effect of tender leaves, high survival rate and low production cost

Inactive Publication Date: 2012-11-21
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the problems such as the high cost of the medium, the easy vitrification and browning of the tissue cultured seedlings, and the long emergence time of the commercial seedlings in the process of the hybrid tissue culture of Populus euramericana, and provide a new The European and American aspen hybrid tissue culture method has the advantages of low production cost, fast seedling growth, strong seedlings, not easy to vitrify, and high survival rate.

Method used

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  • Tissue culture rapid propagation method of populus tremula multiplied by P. tremuloides
  • Tissue culture rapid propagation method of populus tremula multiplied by P. tremuloides
  • Tissue culture rapid propagation method of populus tremula multiplied by P. tremuloides

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Embodiment 1

[0062] (1) Material handling

[0063] After hydroponics, the young stems and new leaves were inoculated from the branches of Populus euramerican hybrids. Before inoculation, they were rinsed with clean water for 5 minutes and 3 to 4 times with sterile water, and then soaked in 75% alcohol for 30 seconds, shaking while soaking. Then sterilized with 0.1% mercuric chloride for 2 minutes, washed 4 times with sterilized distilled water, inoculated on WPM+6-BA1.0mg / L+2% marshmallow medium to start it, and formed sterile test tube seedlings.

[0064] (2) Cultivation operation process

[0065] Differentiation medium: use tap water instead of distilled water to prepare the medium; ② WPM (remove inositol) + 6-BA 1.0 mg / L + KT 0.5 mg / L + 2% commercial soft white sugar (prepared with tap water).

[0066] 1. Differentiation and subculture

[0067] Sterilize the ultra-clean bench for 30 minutes with a UV lamp. All operations in this process were carried out in a sterile environment in a ...

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Abstract

The invention discloses a tissue culture rapid propagation method of populus tremula multiplied by P. tremuloides, which comprises the following steps: (1) inoculating an explant of the populus tremula multiplied by P. tremuloides on a start culture medium for culture after sterilization and obtaining an aseptic tube seedling; (2) cutting the tube seedling obtained in the step (1) into sections, and inserting into a differentiation culture medium for culture; (3) inserting the seedling sections obtained in the step (2) into a rooting culture medium for carrying out rooting culture; (4) transferring the seedling sections after rooting into the rooting culture medium for successive transfer culture; and (5) finally carrying out acclimatization and transplantation. The tissue culture rapid propagation method of populus tremula multiplied by P. tremuloides has the advantages of rapid seedling growth rate, high survival rate, robust seedling, tender green leaf color, difficult vitrification, low production cost, industrialized production and the like.

Description

technical field [0001] The invention relates to a rapid propagation method of aspen hybrids, in particular to a tissue culture rapid propagation method of aspen hybrids, and belongs to the field of tissue culture of aspen hybrids. Background technique [0002] Populus tremula×P.tremuloides is a hybrid of Populus tremula×P.tremuloides, which is a hybrid of Populus tremula×P.tremuloides. It has the characteristics of fast growth, cold resistance, barren tolerance, tall tree shape, and strong adaptability. species. At the same time, the material of the hybrid is soft, dense, white, and the trunk is straight. It is a high-quality raw material for papermaking, plywood, particleboard and other industries. [0003] Studies have shown that: in Finland, European and American aspen hybrids are suitable for mountain afforestation, and plain afforestation grows better, and its rotation period and growth speed are faster than general mountain coniferous and broad-leaved afforestation sp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 杨传平魏志刚闫绍鹏孟凡娟王秋玉
Owner NORTHEAST FORESTRY UNIVERSITY
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