miRNA-n3 derived from rice and application thereof

Abstract

The invention discloses miRNA-n3 derived from rice and application thereof. The miRNA-n3 protected by the invention is RNA shown in a sequence 1 of a sequence table. The pre-miRNA-n3 protected by the invention is the RNA shown in a sequence 2 of the sequence table. The invention also protects the application of the RNA shown in the sequence 1 in inhibiting the gene expression of bHLH transcription factors and / or promoting the degradation of mRNA of the bHLH transcription factors, wherein the bHLH transcription factor is shown in a sequence 3 of the sequence table. By using the miRNA-n3, the plant which has important phenotypes in the aspects of growth and development as well as adverse situation stress tolerance is expected to be obtained, thereby having important biological significancesand potential application value, providing valuable gene resources for the culturing of rice improved varieties (for example culturing of stress-resistant rice), having important research value and potential social benefits and being applied to the production practice finally.

Description

miRNA-n3 derived from rice and its application technical field The present invention relates to miRNA-n3 derived from rice and its application. Background technique miRNA (microRNA, microRNA) is a kind of endogenous single-stranded non-coding small molecule RNA with a length of about 20-24nt, which exists widely in organisms (Bartel D P. MicroRNAs: genomics, biogenesis, mechanisms, and function. Cell , 2004, 116: 281-297.). In recent years, a large number of studies have shown that miRNA can regulate the expression of many genes in organisms, and play an important role in regulating growth and development, cell proliferation, apoptosis, and resisting environmental stress (Bushati N, Cohen S M.MicroRNA functions.Annu. Rev.Cell Dev.Biol., 2007, 23:175-205.; Jin Longguo, Wang Chuan, Liu Jinyuan. Plant MicroRNA. Chinese Journal of Biochemistry and Molecular Biology, 2006, 22:609-614.). Plant miRNA mainly regulates the growth and development of plants and affects their physio...

AI Technical Summary

Problems solved by technology

At present, a considerable number of rice miRNA genes have been discovered, but the number of rice miRNA genes discovered is only a part of the total number of miRNA genes in the organism. Conserved, expression is characterized by low abundance, tissue-specific or induction-specific

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