Method for inoculating tomato yellow leaf curl virus by using injector to inject

Abstract

The invention provides a method for fast inoculating a tomato yellow leaf curl virus and belongs to the field of plant protection. The tomato yellow leaf curl virus is fast inoculated by a stem injection method by using built infectious clone PTYj01. The tomato yellow leaf curl virus is frequently inoculated by the conventional bemisia tabaci gennadius inoculation method in which the bemisia tabaci gennadius needs to be specially fed, and the inoculation efficiency is influenced by the virus transmitting efficiency of the desmone insect and is interfered by the virus transmitting of other bemisia tabaci. The method effectively overcomes the defects of the conventional bemisia tabaci gennadius inoculation method, and has the advantages of no need of feeding the bemisia tabaci gennadius, simple operation, high efficiency, high repeatability, and high controllability, and is a simple and convenient and effective inoculation method.

Description

technical field [0001] The invention relates to an inoculation method of tomato yellow leaf curl virus, which belongs to the field of plant protection. Background technique [0002] Tomato yellow leaf curl is a devastating viral disease on tomato. Since the disease was first reported in 1964, it has been reported in Europe, Africa, Asia, the Caribbean and Oceania, especially in recent years. Recently, with the global warming and changes in farming systems and the increase in international trade, tomato yellow leaf curl has spread globally, causing serious losses to tomato production. In 1991-1992, the disease broke out on tomatoes in Florida, USA, causing losses of 140 million US dollars. [0003] In my country, the tomato yellow leaf curl disease caused by tomato yellow leaf curl virus (Tomato yellow leaf curl virus, TYLCV) was first reported in Shanghai and Zhejiang in 2006. After the tomato is infected by it, the leaf margin will appear early. Yellowing, rolling up, leav...

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Problems solved by technology

However, because the method requires Bemisia tabaci to transmit the virus in the operation process, there are some problems that are difficult to overcome in this method no matter whether it is indoor or in field experiments: 1. The amount of inoculated insects of Bemisia tabaci is difficult to control. The life cycle of whitefly is very short, and the phenomenon of overlapping generations is very serious, resulting in a very strong population proliferation ability, and the individual of whitefly is very small, so whether it is in the field or indoors, the number of whitefly inoculated on a single plant Difficult to control; 2, the number of inoculation of poisonous whitefly is difficult to control, because the amount of inoculated insects of whitefly itself is difficult to control, it also causes the amount of inoculated insects of poisonous whitefly to be difficult to measure; 3, whitefly It is difficult to control the virus transmission efficiency of Bemisia tabaci, because Bemisia tabaci is a kind of poison-transmitting insect, and it can only realize the virus transmission in the process of feeding, and its feeding is affected by many factors, such as temperature, humidity, light, host, etc. , these factors will affect the frequency and amount of feeding, which will affect the inoculation efficiency and the accuracy of the experimental results. When yellow leaf curl virus is detected, whiteflies may carry other viruses at the same time, which will affect the inoculation results

[0005] In addition to the whitefly inoculation method, there are some other inoculation methods, such as grafting inoculation and biolistic inoculation, but these inoculation methods also have problems such as cumbersome operation and high cost.

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