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Tetraiodothyroxide test kit and use method thereof

A detection kit, thyroxine technology, applied in chemical instruments and methods, measuring devices, instruments, etc., can solve the problems of expensive instruments, complex automation design, etc.

Active Publication Date: 2010-10-20
BEYOND DIAGNOSTICS (SHANGHAI) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the separation and cleaning involved in the reaction process, the automation design is complicated and the equipment is quite expensive
In addition, the stability of the luminescent matrix is ​​also a big problem

Method used

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  • Tetraiodothyroxide test kit and use method thereof
  • Tetraiodothyroxide test kit and use method thereof
  • Tetraiodothyroxide test kit and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0111] Example 1 Preparation of T3-coated luminescent particles

[0112] Preparation method of antibody and luminescent particles:

[0113] 1) Suspension treatment of luminescent particles: absorb a certain amount of carboxyl luminescent particles and centrifuge in a high-speed refrigerated centrifuge, discard the supernatant, add a certain amount of MES buffer, ultrasonically break until the particles are resuspended, add MES buffer to adjust the luminescent particles Concentration to 100mg / ml.

[0114] 2) Antibody treatment: T3 was dialyzed against 0.05M MES buffer at pH 6.0 (hereinafter referred to as MES buffer). After the dialysis was completed, the concentration was measured and adjusted to 2 mg / ml.

[0115] 3) Mix MES buffer, 100 mg / ml luminescent particle suspension (MES buffer) and 2 mg / ml T3 (MES buffer) at a volume ratio of 1:10:1, and mix quickly to obtain a reaction solution .

[0116] 4) Prepare 40mg / ml EDAC solution with MES buffer, add according to the ratio...

Embodiment 2

[0182] Example 2 Preparation of biotin-labeled antibody

[0183] Preparation:

[0184] 1) Antibody treatment: dialyze the anti-T4 monoclonal antibody (labeled) in 0.1M NaHCO3 solution, measure the antibody concentration and adjust it to 1mg / mL;

[0185] 2) Prepare 16.17mg / mL Biotin solution with DMSO;

[0186] 3) Labeling: Take the processed 1mg / mL anti-T4 monoclonal antibody (labeled) and the prepared Biotin solution, mix them according to the volume ratio of 10000:54, mix quickly; stand at 2-8°C for reaction 12-16 hours;

[0187] 4) Dialysis: dialyze the reacted biotin-labeled antibody into biotin-labeled dialysis buffer;

[0188] 5) Aspirate and transfer the dialyzed biotinylated antibody to a clean centrifuge tube, take a sample to measure the antibody concentration, and adjust the biotinylated antibody concentration to 0.5 mg / mL with biotinylated dialysis buffer.

[0189] The detection method of each parameter involved in the present embodiment is as follows:

[0190...

Embodiment 3

[0214] Example 3 Preparation of Photosensitive Microparticles Coated with Avidin

[0215] Photosensitive particles: use photosensitive particles with a particle size of 220±40nm (PentaTek, USA)

[0216] Preparation:

[0217] a. Treatment of photosensitive particle suspension: absorb a certain amount of photosensitive particles and centrifuge in a high-speed refrigerated centrifuge, discard the supernatant, add a certain amount of MES buffer, ultrasonically sonicate on the ultrasonic cell disruptor until the particles are resuspended, and then add MES buffer Adjust the photosensitive particle concentration to 100mg / ml.

[0218] b. Preparation of avidin solution: weigh a certain amount of Avidin, add MES buffer to dissolve to 8mg / ml.

[0219] c. Mixing: Mix the treated photosensitive microparticle suspension, 8 mg / ml Avidin and MES buffer at a volume ratio of 2:5:1, and mix quickly to obtain a reaction solution.

[0220] d. Reaction: Prepare 25mg / ml NaBH in MES buffer 3 CN s...

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Abstract

The invention relates to an auxiliary diagnostic kit of thyroid diseases, and discloses a tetraiodothyroxide test kit which comprises tetraiodothyroxide testing particles, tetraiodothyroxide resisting antibody marked by biotin and disintegrating agent; wherein triiodothyronine testing particles are luminous particles coated by diiodothyronine, and the disintegrating agent is 8-anilino-1- naphthalenesulfonic acid ammonium salt hydrate. The invention also discloses a use method of a triiodothyronine kit. The kit can be combined with other serum and clinical information for the auxiliary diagnosis of thyroid diseases and the monitoring of the treatment effect of relevant patients.

Description

technical field [0001] The invention relates to a detection kit for tetraiodothyronine, in particular to a detection kit for tetraiodothyronine based on the principle of light-induced chemiluminescence and a use method thereof. Background technique [0002] Thyroid disease is a group of more common endocrine diseases, including iodine deficiency goiter, goiter caused by other reasons, hyperthyroidism, thyroid tumor, thyroiditis, hypothyroidism and so on. The incidence of thyroid disease is relatively high. [0003] Hyperthyroidism, also known as hyperthyroidism, is a very common clinical endocrine disease. It refers to the enhancement of thyroid function caused by various reasons, excessive secretion of thyroid hormones or increased levels of thyronine (T4, T4) in the blood caused by the body's nervous system, circulatory system, digestive system, cardiovascular system, etc. Systemic series of hypermetabolic syndrome and symptoms of hyperexcitability and ocular symptoms. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/542G01N21/64C07C229/36C09K11/02
Inventor 陈晨辉王海蛟赵卫国
Owner BEYOND DIAGNOSTICS (SHANGHAI) CO LTD
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