Method for preparing biodegradable oil residue fertilizer
A biodegradation and oil residue technology, applied in the direction of organic fertilizers, fertilizer forms, fertilizer mixtures, etc., can solve the problems of difficult crushing, hard dry oil residue, affecting the effect of fertilizers, etc., and achieve the effect of good production increase effect.
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[0008] A method for preparing biodegradable oil residue fertilizer, including the preparation of four fermentation broths including Bacillus subtilis fermentation broth, Yarrowia lipolytica fermentation broth, Geotrichum candidum fermentation broth and Lactobacillus plantarum fermentation broth, and is characterized in that:
[0009] (1) The four fermentation broth strains were selected from the China Industrial Microbial Culture Collection and Management Center. The strain catalog numbers are as follows: Bacillus Subtilis No. 10210, Yarrowia lipolytica No. 1444, Geotrichum candidum (Geotrichum candidum) No. 31418, Lactobacillus plantarum (Lactobacillus plantarum) No. 6026;
[0010] (2) Take 100-150 parts of Bacillus subtilis fermentation broth and 100-150 parts of Yarrowia lipolytica fermentation broth according to parts by mass and add them to 1000 parts of oil residue, adjust the water content to 45-55wt%, and the pH to be 6.5-7.0. Mix well, control the temperature of the materi...
specific Embodiment approach
[0035] The present invention will be further described below in conjunction with embodiments, but it is not limited thereto.
[0036] Oil residue is the residue remaining after Shandong Everbright Riyue Co., Ltd. produces oil, including soybean oil residue, peanut oil residue, cottonseed oil residue or rape oil residue.
[0037] The strains used in the examples are: Bacillus Subtilis (Bacillus Subtilis) China Industrial Microorganism Collection Management Center strain catalog number 10210, Yarrowia lipolytica (Yarrowia lipolytica) China Industrial Microorganism Collection Management Center strain catalog number 1444, Baidi Geotrichum (Geotrichum candidum) China Industrial Microorganism Collection Management Center strain catalog number 31418, Lactobacillus plantarum (Lactobacillus plantarum) China Industrial Microorganism Collection Management Center strain catalog number 6026, purchased by China Industrial Microorganism Collection Management Center.
[0038] The raw materials used ...
Embodiment 1
[0040] (1) Preparation of Bacillus subtilis fermentation broth
[0041] Take 200 grams of peeled potatoes, add 1000 grams of water and boil for 20 minutes, filter with gauze, add water to 1000 grams of filtrate, add 15 grams of glucose and 2 grams of peptone, heat until all ingredients are melted, and sterilize at 0.11 MPa for 30 minutes , Prepared into a liquid medium, cultured the activated Bacillus subtilis and Yarrowia lipolytica in a shake flask at 28°C and 180 revolutions per minute for 2 days; then transferred to the medium with a 2% inoculum. The formula was potato 200 Kg, 8 kg glucose, 30 kg oil residue, and 1000 kg water were cultured at 28°C for 4 days to obtain the Bacillus subtilis fermentation broth.
[0042] (2) The preparation method of Yarrowia lipolytica fermentation broth is the same as that of Bacillus subtilis fermentation liquid, except that the Yarrowia lipolytica strain is connected.
[0043] (3) Take 150 kg of Bacillus subtilis fermentation broth and 100 kg ...
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