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Tumor cell vaccine and preparation method thereof

A tumor cell vaccine and tumor cell technology, applied in the field of tumor biotherapy, can solve problems such as less significant research

Inactive Publication Date: 2016-05-25
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, VEGF can also have a chemotactic effect on monocytes through the interaction of the receptor FLT-1 on the surface of monocytes, but there are few studies on this function of VEGF and its significance in tumorigenesis and development.

Method used

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  • Tumor cell vaccine and preparation method thereof
  • Tumor cell vaccine and preparation method thereof

Examples

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Embodiment 1

[0025] The preparation of embodiment one tumor vaccine of the present invention

[0026] Taking mouse LL / 2 lung cancer cells as an example: using LL / 2 mouse lung cancer as an animal model, the mouse VEGF164 eukaryotic expression gene was introduced into LL / 2 tumor cells, and a stable high-expression strain of VEGF (LL / 2) was screened. / 2MV); LL / 2 tumor cell line with high expression of VEGF, after G o 60 inactivation treatment until it has no differentiation and growth activity, but can still survive and have the activity of secreting cytokines. After the inactivated tumor cells were inoculated into C57 mice, the mice were inoculated with parental LL / 2 tumor cells, and the protective effect of the inactivated tumor cell vaccine parental LL / 2 tumor cells was observed.

[0027] Specific operation process:

[0028] 1. Transfection and screening of LL / 2 cell lines highly expressing mVEGF1640

[0029] LL / 2 was cultured in DMEM matrix containing 10% inactivated fetal bovine serum...

Embodiment 2

[0030] Example 2: Tests of tumor prevention and treatment using the tumor vaccine of the present invention

[0031] Fifteen 8-week-old female C57 mice were randomly divided into 3 groups for the prevention test. The logarithmically grown LL / 2MV and LL / 2C-P tumor cells were collected, centrifuged and washed 3 times with serum-free matrix, counted with a hemocytometer, and the number of cells was adjusted to 20×10 6 pcs / ml, use C 0 60 machines were irradiated with a dose of 20Grays for 20 minutes. Treat LL / 2MV and LL / 2C-P with this method and subcutaneously inject 100ul on the back of the mouse respectively (that is, each mouse injects 2×106 radiation-treated tumor cells) each of 5 mice, and the other 5 mice were subcutaneously injected with 100ul serum matrix on the back as a control. At intervals of 15 days, each group was injected once more according to the above method. After an interval of 15 days, the logarithmic growth parental LL / 2 tumor cells were collected and centr...

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Abstract

The invention belongs to the field of tumor biotherapy and relates to a tumor cell vaccine and a preparation method thereof. The technical problem to be solved by the invention is to provide an effective tumor prevention and cure vaccine. To solve the technical problem, the invention adopts a technical scheme of providing the tumor cell vaccine. The tumor cell vaccine uses tumor cells, which are inactivated and lose differentiating and proliferating capability, but can still express a vascular endothelium growth factor and contain vascular endothelium growth factor expression vectors, as main active ingredients.

Description

technical field [0001] The invention belongs to the field of tumor biotherapy, and in particular relates to a tumor cell vaccine and a preparation method thereof. Background technique [0002] As we all know, the immunogenicity of tumor antigens is weak, and it is difficult for the host to produce a specific immune response. Tumor cells can escape the immune surveillance of the body, which is one of the main reasons for the uncontrollable proliferation of tumor cells. Macrophages are widely distributed in various tissues of the body. They are important auxiliary cells in the initial stage of immune response, and also the first barrier for the body to process foreign antigens and immunity. However, how to mobilize phagocytes to effectively obtain tumor antigens, present a large number of tumor antigen epitopes to the surface of macrophages and present them to T cells, thereby effectively stimulating the body's immune response and producing a preventive effect on tumors, has l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/00A61K48/00A61P35/00
Inventor 阚兵魏于全杨莉王永生
Owner SICHUAN UNIV
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