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Han children asthma susceptibility gene detection model and kit thereof

A detection kit and a technology for children's asthma, which are applied in the field of detection models to achieve good detection efficiency

Inactive Publication Date: 2010-09-15
XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Abstract
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  • Application Information

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Problems solved by technology

[0005] However, traditional statistical methods can only conduct combined research on two SNP sites at the same time at most. If a new statistical method that can perform high-order interaction analysis on multiple sites at the same time is found, it may be more ideal. Asthma susceptibility gene detection model

Method used

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  • Han children asthma susceptibility gene detection model and kit thereof
  • Han children asthma susceptibility gene detection model and kit thereof
  • Han children asthma susceptibility gene detection model and kit thereof

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Effect test

Embodiment 1

[0026] 1 Materials and methods

[0027] 1.1 Materials

[0028] 1.1.1 Research object

[0029] (1) Asthma case group: From 2007 to 2008, 192 asthmatic children from the asthma clinic of Xinhua Hospital, half male and half male, aged 3-12 years. All met the diagnostic criteria for childhood asthma revised by the Global Initiative for Asthma (GINA) committee in 2006: frequent wheezing episodes (or traceable to certain allergens or stimulating factors); Wheezing mainly in the gaseous phase, prolonged expiratory phase; bronchodilators have obvious curative effect, and there is reversible airflow limitation; other diseases that cause wheezing, chest tightness and cough are excluded. Because children with RSV infection often suffer from wheezing, the lower age limit for enrollment was 3 years old. 75% of the enrolled children with IgE data had their total IgE levels above 400IU / ml.

[0030] (2) Normal control group: 192 healthy students from Shanghai Normal University and East Ch...

Embodiment 2

[0285] Clinical application of the asthma susceptibility gene detection model in Han children

[0286] The Department of Pediatrics of Xinhua Hospital took 192 case-control cases from the Han population as the research objects, and genotyped 15 single nucleotide polymorphisms (single nucleotide polymorphisms, SNPs) sites in 8 genes. Multifactor dimensionality reduction (MDR), the mainstream statistical method for the correlation between SNP loci, was used for statistical analysis, and a preliminarily established FcER1 E237G, FcER1 C-109T, ADRB2R16G, IL4RA Q551R, IL4RA I75V, IL4C -590T, IL13 A2044G, IL13 C1923T and IL13 C-1112T nine SNP loci for the detection of asthma susceptibility genes in Han children, which has good asthma detection efficiency (OR=345.3478, P value<0.0001, accuracy 92.97% , sensitivity 97.92%, specificity 88.02%). The establishment of the asthma susceptibility gene detection model of Han children is the first in China, and it is innovative; compared with ...

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Abstract

The invention relates to a Han children asthma susceptibility gene detection model which consists of nine SNP loci including FcER1 E237G, FcER1 C-109T, ADRB2 R16G, IL4RA Q551R, IL4RA I75V, IL4 C-590T, IL13 A2044G, IL13 C1923T and IL13 C-1112T. The invention also provides a Han children asthma susceptibility gene detection kit. The invention has the advantages that the Han children asthma susceptibility gene detection model is established for the first time in China, thus having innovation; and compared with foreign similar research, the Han children asthma susceptibility gene detection model has species specificity. The Han children asthma susceptibility gene detection kit which detects the nine SNP loci has good asthma detection efficiency (OR=345.3478, P value is less than 0.0001, the accuracy is 92.97%, the sensitivity is 97.92%, and the specificity is 88.02%).

Description

【Technical field】 [0001] The invention relates to a detection model, in particular to a detection model of asthma susceptibility gene of Han children and a kit thereof. 【Background technique】 [0002] Bronchial asthma is one of the most common chronic airway inflammatory diseases in the world today. It is also a common and frequently-occurring disease of children's respiratory system. Its morbidity and mortality are increasing year by year, causing serious harm to children's health and society. In recent years, with the continuous development of molecular biology and genetics, research on asthma genes has become increasingly in-depth. It is currently recognized that asthma has an obvious tendency to cluster in families and is a complex multi-factor and multi-gene genetic disease. [0003] Finding the susceptibility genes of asthma on the human genome is a hotspot in the study of disease susceptibility in the post-genome era. The year 2006 is the 10th year since the screenin...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 鲍一笑华丽
Owner XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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