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Method for fast identifying harmful algae

A red tide algae, rapid technology, applied in the direction of biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of loss of offshore aquaculture, hindered respiration, etc., and achieve the effect of rapid and simple preliminary identification

Inactive Publication Date: 2010-09-08
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Finally, the mucus produced by some red tide organisms (such as certain dinoflagellates) adheres to the gills of marine animals, hinders respiration, suffocates large aquatic animals, and causes huge losses to the offshore aquaculture industry

Method used

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  • Method for fast identifying harmful algae
  • Method for fast identifying harmful algae
  • Method for fast identifying harmful algae

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Experimental program
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Embodiment Construction

[0072] The invention provides a method for rapid identification of red tide algae species, which comprises the following steps:

[0073] a. Preparation of experimental algal species f / 2 and its derivative medium

[0074] b. Primer design and verification

[0075] c. Obtaining 18S rDNA fragments of experimental algae species

[0076] d. PCR optimization and amplification

[0077] e. Simulated enzyme digestion and actual enzyme digestion control experiment

[0078] f. RFLP distinguishes and identifies red tide algae species.

[0079] Described step a is:

[0080] f / 2 Trace element solution: Add ingredients to 950mL double distilled water: FeCl3·6H2O, Na2EDTA·2H2O, CuSO4·5H2O, Na2MoO4·2H2O, ZnSO4·7H2O, CoCl2·6H2O, MnCl2·4H2O, set the volume to 1L, extinguish at high temperature bacteria. ;

[0081] f / 2 vitamin solution: add 200mg vit.B1 to 950mL distilled water, add 1ml vit.H and vit.B12 storage solution, dilute to 1L, filter and sterilize, and place at 4°C;

[0082] f / 2 ...

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Abstract

The invention discloses a method for fast identifying harmful algae. The method is characterized by comprising the following steps of: a, preparation of experimental algae f / 2 and derivative culture media of the experimental algae; b, design and verification of primers; c, obtaining of 18s rDNA fragment of the experimental algae; d, optimization and propagating of (polymerase chain reaction) PCR; e, control experiment of simulated enzyme cutting and practical enzyme cutting; and f, identification of the harmful algae by using restriction fragment length polymorphism (RFLP). As a molecule ecological means for identifying the harmful algae, the method can serve as the supplementary means and auxiliary means of the conventional identification methods. The method establishes a characteristic map based on FELP, standardizes the whole flow, and is favorable for initially identifying the harmful algae fast, easily and conveniently and pre-alarming the outbreak of the red tide of phytoplankton.

Description

Technical field: [0001] The invention relates to the technical field of marine phytoplankton species and / or quantity detection, in particular to a method for rapid identification of red tide algae species. Background technique: [0002] In the rich ocean world, there are many marine creatures. In addition to various marine fish and other marine animals, there are also marine plants that provide basic productivity for the ocean. They are distributed in all levels of the ocean, and marine algae, which are the main components of marine plants, are a precious natural wealth of human beings. They can be used as food and health medicines. According to the different living habits of marine algae, people divide them into two types: benthic algae and planktonic algae. [0003] Phytoplankton consists of only one cell, also known as unicellular algae, which is a type of autotrophic unicellular plankton that has pigments or pigment bodies that can perform photosynthesis and produce org...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04
Inventor 张涛甄毓米铁柱于志刚
Owner OCEAN UNIV OF CHINA
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