Method for purifying protopine from corydalis amabilis

A purification method and proto-opioid technology, applied in ultrafiltration, organic chemistry and other directions, can solve the problems of many toxic reagents and low resin specificity, and achieve the effects of increasing speed, reducing loss and increasing adsorption capacity.

Inactive Publication Date: 2010-08-18
NANJING ZELANG MEDICAL TECH
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AI Technical Summary

Benefits of technology

This technology helps improve the efficiency of separating materials by removing unwanted substances from them while also increasing their ability to absorb certain compounds or molecules that are important for biological processes like cell growth. It can be applied without requiring activation treatment with an expensive chemical agent such as acids or bases. Mixed resins have improved performance compared to pure polymers but they still lose effectiveness when used alone due to factors like high levels of impurity content.

Problems solved by technology

This patented describes various techniques for obtaining pure compounds called probovopsins by isolating certain molecules found naturally occurring within herbs like papyrus flowers. These processes involve treatments with different chemical agents at very small concentrities and can lead to side effects on other organs involved during their production process. Therefore, it would be useful if these procedures were improved over time without increasing safety concerns associated with potentially harmful substances.

Method used

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  • Method for purifying protopine from corydalis amabilis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Step 1: Extract. Weigh 20 kg of Xia Wu (original opium content 0.4%) and grind to 20 meshes, add 120L85% ethanol to reflux for extraction for 2 hours, filter, add 100L85% ethanol, reflux for 1 hour, filter, then add 100L85% ethanol, reflux for 1 hour; combine the three extracts to obtain 280L.

[0034] The second step: membrane separation. Concentrate the extract under reduced pressure until the alcohol concentration is 40%, and release it to obtain 80L of concentrated solution. Slowly add 240ml of concentrated acetic acid, fully stir, filter, add a microfiltration membrane system to filter, and then pass through a composite ultrafiltration membrane with a molecular cut-off of 3000. Ultrafiltration, Collect permeate.

[0035] The third step: mixed resin enrichment. The permeate was added to 10LAB-8 and 110 resin 3:1 mixed resin column for adsorption, the column flow rate was 5L / hour. During elution, first wash the neutral with deionized water, then use 60L of 50% et...

Embodiment 2

[0038] Step 1: Extract. Weigh 20 kg of Xia Wu (original opium content 0.4%) and pulverize into 40 meshes, first add 160L of 90% ethanol for reflux extraction for 2 hours, filter, then add 120L of 90% ethanol, reflux for 1 hour, and combine the extracts to obtain 240L .

[0039] The second step: membrane separation. Concentrate the extract under reduced pressure until the alcohol concentration is 30%, and release it to obtain 70L of concentrated solution, slowly add 140ml of concentrated acetic acid, stir well, filter, add microfiltration membrane system to filter, and then pass through a composite ultrafiltration membrane with a molecular cut-off of 3000. Collect permeate.

[0040] The third step: mixed resin enrichment. The permeate was added to 10LAB-8 and 110 resin 2:1 mixed resin column for adsorption, the column flow rate was 5L / hour. During elution, first wash the neutral with deionized water, then use 50L of 60% ethanol solution to elute impurities, and finally use ...

Embodiment 3

[0043] Step 1: Extract. Weigh 10 kg of Xia Wu (0.45% of the original opioid content) and pulverize into 20 meshes, first add 60L of 90% ethanol for reflux extraction for 2 hours, filter, then add 50L of 90% ethanol, reflux for 1 hour, filter, then add 50L of 90% ethanol was refluxed for 1 hour; the three extracts were combined to obtain 140L.

[0044] The second step: membrane separation. Concentrate the extract under reduced pressure until the alcohol concentration is 30%, and release it to obtain 40L of the concentrated solution, slowly add 40ml of concentrated acetic acid, stir thoroughly, filter, add a microfiltration membrane system to filter, and then pass through a composite ultrafiltration membrane with a molecular cut-off of 6000. Collect permeate.

[0045]The third step: mixed resin enrichment. The permeate was added to 5LAB-8 and 110 resin 3:1 mixed resin column for adsorption, the column flow rate was 5L / hour. During elution, first wash the neutral with deioniz...

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Abstract

The invention relates to a protopine extraction purification method with simple operation and low cost. The technology includes that corydalis amabilis medicinal material with high content of protopine is taken as raw material, smashing is carried out, ethanol reflux extraction is carried out for 2-3 times, reduced pressure concentration is carried out to low alcohol and acetic acid is added for fluxing, compound ultrafiltration membrane is used for holding back macromolecule impurity, then mixed resin is used for enrichment, gradient elution is carried out for removing impurities, protopine eluent is collected, ammonia water is used for regulating pH value, crystallization is carried out, crude crystal is separated, ethanol and ethanol chloroform are used for carrying out step recrystallization. By adopting the method to produce product, period is short, and yield is high. The technology is easy to be amplified and industrialized.

Description

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Claims

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Application Information

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Owner NANJING ZELANG MEDICAL TECH
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