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A kind of oocyte/embryo vitrification cryopreservation method and cryopreservation carrier thereof

A vitrification and oocyte technology, applied in the field of oocyte/embryo vitrification cryopreservation method and its freezing carrier, can solve the problems of not being suitable for beginners and difficult to operate, and avoid high cost and cumbersome Procedures, easy storage, and the effect of a wide range of materials

Inactive Publication Date: 2012-02-22
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantage of this method is that the semi-thin tube makes the freezing liquid directly contact with liquid nitrogen, which greatly improves the freezing speed, and has the advantage of being easy to store; the disadvantage is that the operation is difficult, and skilled technicians are required, so it is not suitable for beginners

Method used

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  • A kind of oocyte/embryo vitrification cryopreservation method and cryopreservation carrier thereof
  • A kind of oocyte/embryo vitrification cryopreservation method and cryopreservation carrier thereof

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Embodiment Construction

[0021] The present invention will be further elaborated below by specific examples.

[0022] (1) EFS30-40 vitrification storage solution, freezing pretreatment solution and thawing solution.

[0023] ① EFS30~40 vitrification storage solution: take PBS as the base solution, add polysucrose, sucrose and ethylene glycol, and prepare polysucrose with a volume ratio of 30%, sucrose with a concentration of 0.5mol / L, and ethylene glycol with a volume ratio of 30%. It is 30%-40% EFS30-40 cryopreservation solution.

[0024] ② The freezing pretreatment solution is 5% to 10% ethylene glycol (Ethylene glycol: EG): PBS is used as the base solution, and ethylene glycol is added to prepare a 5% to 10% ethylene glycol freezing pretreatment solution by volume. treatment fluid.

[0025] ③ The thawing solution is 0.3-0.5 mol / L sucrose solution: use PBS as the base solution to prepare 0.3-0.5 mol / L sucrose solution as the thawing solution.

[0026] (2) Freezing: At room temperature (20-25°C), ...

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Abstract

The invention relates to a preservation method for human body, animal or its part and its freezing carrier, in particular to a vitrification freezing preservation method of oocyte / embryo and its freezing carrier. The method comprises: a freezing liquid preparation step, a thawing liquid preparation step, a freezing step and a thawing step, wherein the freezing carrier used in the freezing step and the thawing step is a freezing net, and the freezing net comprises: a mesh body and net handle. The invention provides a simple, fast and efficient oocyte or embryo vitrification freezing method and its freezing carrier.

Description

technical field [0001] The invention relates to a preservation method for human body, animal or its part and its freezing carrier, in particular to a vitrification freezing preservation method of oocyte / embryo and its freezing carrier. Background technique [0002] Since Rall et al. (1985) invented the embryo vitrification cryopreservation method, the cryopreservation can be performed at room temperature without the aid of a freezer, and the procedure has been greatly simplified. After improvement by many researchers, the current oocyte / embryo vitrification method only takes about 1 minute to complete a procedure. During this process, many researchers creatively invented many frozen carrying objects, which were named in various ways. The following methods are commonly used today: Nylon Ring Vitrification (Cryoloop), Open Pulled Straw (OPS, Open Pulled Straw), Fine Glass Tube (GMP), Microdrops, Semi-thin Tube Law (hemi-straw). [0003] Nylon ring vitrification method (Cryo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 朱士恩李俊杰王彦平侯云鹏
Owner CHINA AGRI UNIV
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