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Callus Induction method of anther of small fruit tomatoes

A technology of callus induction and callus, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as the external morphology research of tomato anthers, and achieve the goal of improving callus induction rate and accuracy Effect

Inactive Publication Date: 2010-07-14
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this prior art has not done in-depth research on the external morphology of tomato anther culture at the optimum time for material extraction.

Method used

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  • Callus Induction method of anther of small fruit tomatoes
  • Callus Induction method of anther of small fruit tomatoes
  • Callus Induction method of anther of small fruit tomatoes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] In this embodiment, three small-fruited tomato varieties were selected: Huangluan, Ruizhen small tomato, and Gaonong mating.

[0026] The first step is to pick the flower buds of several small-fruited tomatoes at the same time, put them into standard fixative solution for fixed preservation after being sorted by length.

[0027] The second step is to observe the external morphology of different flower bud lengths (3-12 mm, with an interval of 1 mm) of small-fruited tomatoes, and study the various developmental stages of pollen (microspore mother cell stage, tetrad stage, early mononucleate stage, and mid-uninucleate stage) , uninucleate marginal stage, dinuclear stage, mature pollen grain stage) cytological characteristics.

[0028] By studying the cytological characteristics of tomato pollen at each developmental stage, it was found that the flower bud length was positively correlated with the pollen development stage in tomato small fruit, and the external morphology ...

Embodiment 2

[0034] Three small-fruited tomato varieties were used in this example: Victory 189, Gaonong Mating, and Huangluan.

[0035] The first step is to pick the flower buds of several small-fruited tomatoes at the same time, put them into standard fixative solution for fixed preservation after being sorted by length.

[0036] The second step is to observe the external morphology of different flower bud lengths (3-12 mm, with an interval of 1 mm) of small-fruited tomatoes, and study the various developmental stages of pollen (microspore mother cell stage, tetrad stage, early mononucleate stage, and mid-uninucleate stage) , uninucleate marginal stage, dinuclear stage, mature pollen grain stage) cytological characteristics.

[0037] By studying the cytological characteristics of tomato pollen at each developmental stage, it was found that the flower bud length was positively correlated with the pollen development stage in tomato small fruit, and the external morphology of flower buds al...

Embodiment 3

[0043] In this example, three small-fruited tomato varieties were selected: Victory 189, Gaonong Mating, Xianfeng×Qismani.

[0044] The first step is to pick the flower buds of several small-fruited tomatoes at the same time, put them into standard fixative solution for fixed preservation after being sorted by length.

[0045] The second step is to observe the external morphology of different flower bud lengths (3-12 mm, with an interval of 1 mm) of small-fruited tomatoes, and study the various developmental stages of pollen (microspore mother cell stage, tetrad stage, early mononucleate stage, and mid-uninucleate stage) , uninucleate marginal stage, dinuclear stage, mature pollen grain stage) cytological characteristics.

[0046] By studying the cytological characteristics of tomato pollen at each developmental stage, it was found that the flower bud length was positively correlated with the pollen development stage in tomato small fruit, and the external morphology of flower...

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Abstract

The invention relates to a callus induction method of anther of small fruit tomatoes in the field of agricultural breeding technology. Steps of this method are demonstrated as follows. First, pick up the buds from a number of small fruit tomatoes at one time, classify them in terms of length, and preserve them in standard fixed fluid. Then take out pollens at the mid-late mononuclear stage from small fruit tomatoes and sterilize them. After that pick up anther from buds in an sterilized environment, and inoculate them with culture medium. Finally, cultivate the inoculated anther in dark so as to obtain calluses of small fruit tomatoes. In this invention, the key to successfully obtaining calluses lies in the identification of the developmental stages of pollens. Since the callus induction rates vary in terms of different lengths of buds, and by the comparison of the development stages of the pollen, the best cultivation stage of the small fruit tomatoes can be identified.

Description

technical field [0001] The invention relates to a method in the technical field of agricultural breeding, in particular to a method for inducing callus of small-fruited tomato anthers. Background technique [0002] Small-fruited tomato belongs to Solanaceae (Solanaceae) tomato genus (lycopersicon) semi-cultivated subspecies of tomato, which is rich in nutrition and unique in flavor. In recent years, with the adjustment of industrial structure and the increase of consumer demand, its planting area has developed rapidly, and it occupies an increasingly important position in the market supply. Anther culture is a very effective method in tomato haploid breeding. Combining chromosome doubling can save multiple generations of selfing, quickly establish and breed pure tomato lines, overcome the segregation of hybrid offspring traits, and speed up the process of tomato breeding. [0003] After searching the prior art, it was found that Zhu Liping and others have done some research...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘杨王芹芹韩洪强陈火英
Owner SHANGHAI JIAO TONG UNIV
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