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Detection method of cordycepic acid content in cordyceps product

A detection method, the technology of cordycepin, is applied in the direction of material analysis by observing the effect on chemical indicators, and analysis by making the material undergo chemical reaction. It can solve errors, affect the stability and reproducibility of results, and only once It can detect problems such as a sample, and achieve the effect of low detection limit, low equipment price, simple and practical detection method

Inactive Publication Date: 2012-10-10
AFFILIATED HOSPITAL OF ZUNYI MEDICAL COLLEGE
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Problems solved by technology

However, the UV-Vis spectrophotometer can generally measure up to 3 samples at the same time. If each test sample needs to be set for 3 repetitions, only one sample can be tested at a time.
Therefore, the quantitative analysis of batch samples is subject to errors caused by the detection system and human factors, which seriously affects the stability and reproducibility of the results.

Method used

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  • Detection method of cordycepic acid content in cordyceps product

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Embodiment Construction

[0023] The method for detecting the content of cordycepic acid in the Cordyceps product of the present invention will be further described in detail below in conjunction with the accompanying drawings and examples, but it is not used as a basis for any limitation of the present invention.

[0024] Example. A method for detecting the content of cordycepic acid in cordyceps products. The method is to first make a certain amount of cordyceps products into a test solution according to a conventional method; then make the test solution into a test solution, and use a microplate reader to detect the absorbance of the test solution ; Cordycepic acid was prepared into 6 concentrations of the standard solution, and its absorbance was measured.

[0025] The standard curve of cordycepic acid was established with the standard solution concentration as the abscissa and the absorbance value as the ordinate. The values ​​of 6 standard solutions 10mg / L, 20mg / L, 30mg / L, 40mg / L, 50mg / L, 60mg / L...

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Abstract

The invention discloses a detection method of cordycepic acid content in a cordyceps product. The method comprises the following steps: firstly using the cordyceps product to prepare a solution to be detected through a conventional method, secondly using the solution to be detected to prepare a test solution, detecting the absorbance of the test solution with a microplate reader; using cordycepicacid to prepare 5-7 standard solutions with different concentrations, measuring the absorbance of each standard solution; drawing a standard curve of cordycepic acid by using the concentrations of the standard solutions as abscissa and using the absorbance values as ordinate, establishing a corresponding linear relationship equation between the concentration of cordycepic acid and the absorbance,then substituting the absorbance value of the test solution in the equation to obtain the content of cordycepic acid of the test solution and performing conversion operation to obtain the weight percentage of cordycepic acid in unit mass of the cordyceps product. The invention adopts the simple method and cheap devices to detect the cordycepic acid contents of a great number of cordyceps productssimultaneously, the system error and the accidental error are reduced, the detection result is not easy to be affected by the external interference and the method of the invention is applicable to conventional quantitative analysis.

Description

technical field [0001] The invention relates to a method for detecting the content of cordycepic acid in cordyceps products. Background technique [0002] Cordycepic acid, namely mannitol, is one of the main active ingredients in Cordyceps sinensis and other traditional Chinese medicines of Cordyceps sinensis. Multiple effects. As for the detection method of cordycepic acid content in Cordyceps sinensis and its related products, there are many methods including volumetric method, UV-visible spectrophotometry, high performance liquid chromatography (HPLC), gas chromatography (GC) and capillary zone Electrophoresis (CZE), etc. According to the Chinese Pharmacopoeia in 2005, the content of cordycepic acid is quantitatively analyzed by volumetric method. The volumetric method is based on the reduction of mannitol and uses the oxidant periodate to titrate and calculate the content. Due to the interference of reducing substances in Cordyceps sinensis and other Cordyceps fungus ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
Inventor 肖建辉钟建江陈代雄肖代敏
Owner AFFILIATED HOSPITAL OF ZUNYI MEDICAL COLLEGE
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