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Bacteriophage EK99-C of enterotoxigenic Escherichia coil ETEC-K99 and application thereof

A technology of EK99-C and Escherichia coli, applied in the field of bacteriophage, can solve the problems of high incidence of piglet diarrhea and the inability to effectively control the spread of the disease, achieving obvious effects, preventing overgrowth, and strong specificity

Inactive Publication Date: 2012-05-09
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0016] Another object of the present invention is to provide a new treatment product and treatment means for the high incidence of piglet diarrhea caused by enterotoxigenic E. A new single or combined drug product and treatment method with significant therapeutic effect on diarrhea caused by ETEC-K99 in piglets, lambs, and calves

Method used

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  • Bacteriophage EK99-C of enterotoxigenic Escherichia coil ETEC-K99 and application thereof
  • Bacteriophage EK99-C of enterotoxigenic Escherichia coil ETEC-K99 and application thereof
  • Bacteriophage EK99-C of enterotoxigenic Escherichia coil ETEC-K99 and application thereof

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Embodiment 1

[0056] Example 1: Isolation and preparation of bacteriophage EK99-C

[0057] The phage host bacterium ETEC-K99 used in the experiment of the present invention is a host bacterium isolated and identified in a pig farm by the Veterinary Research Institute of Jiangsu Academy of Agricultural Sciences.

[0058] The sample of the present invention is collected from the sewage of Jingrong pig farm in Sheyang, Jiangsu Province, filtered through double-layer filter paper, centrifuged at 3000rpm for 20min, and then filtered with a 0.22μm filter membrane to supernatant.

[0059] Take 50ml filter supernatant, add 2ml phage host bacteria ETEC-K99 overnight culture, then add CaCl 2 After mixing the mother solution (sterilized by a 0.22 μm filter membrane) to a final concentration of 1 mM, add 20 ml of LB medium, incubate at room temperature for 1 h, and then place it at 37 ° C for 12 to 16 h. The next day, the above culture was centrifuged at 14000rpm for 30min, and the supernatant was tak...

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Abstract

The invention relates to a bacteriophage, in particular to a strong-lytic bacteriophage EK99-C of an enterotoxigenic Escherichia coil K99 (ETEC-K99). The invention discloses the strong-lytic bacteriophage of the enterotoxigenic Escherichia coil K99 obtained by separation and application thereof in the aspects of young animal diarrhoea treatment, bacterial infection and environment purification and sterilization. The bacteriophage (named EK99-C) obtained by separation is a bacteriophage monomer which has strong lytic activity to a pathogenic E. coli K99 bacterial strain (ETEC-K99) and can be applied to the treatment of animal diarrhoea caused by the K99 bacterial strain of young animals (such as pigs, cattle and sheep), sterilization of environments of animal house breeding, slaughtering and the like, and the sterilization of the K99 bacterial strain on apparatuses. The invention also provides a new method and a new idea for treating the animal diarrhoea and sterilizing the environments and the apparatuses, namely biological prevention and control technology. The bacteriophage is preserved in the CCTCC and the preservation number is CCTCC NO: M209208.

Description

field of invention [0001] The present invention relates to a phage, in particular to a strong lytic phage EK99-C of enterotoxigenic Escherichia coli K99 (ETEC-K99), its isolate and its single or mixed use are used in the treatment of young animal diarrhea and anti-bacterial infection And the application of environmental purification and sterilization. Background of the invention [0002] Enterotoxigenic E.coli (Enterotoxigenic E.coli, ETEC) is one of the common and important pathogenic bacteria in young animals (piglets, calves, lambs, etc.) Pili antigens adhere to the small intestinal mucosa of animals, colonize and produce enterotoxin, thus presenting a pathogenic effect. The pathogenicity of Escherichia coli is mainly composed of two types of virulence factors: adhesin pili and enterotoxin. At present, the pili antigens of Escherichia coli that have been found and studied in various animals mainly include: K88(F4), K99(F5), 987P(F6), F41 and F17. In enterotoxigenic Esc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A61K35/76A61P1/12A61P31/04A61L2/00A61L9/00A61L101/52
Inventor 王冉张辉包红朵魏瑞成
Owner JIANGSU ACAD OF AGRI SCI
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