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A kind of Paenibacillus macerans for preventing and treating plant bacterial wilt and its application

A technology of bacillus and hemp, applied in the field of Paenibacillus, can solve the problems of damage to the host vascular bundle conduction tissue, easy loss of resistance, low resistance, etc., and achieve the effect of preventing and treating plant bacterial wilt

Inactive Publication Date: 2011-12-07
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although different plants show some differences in symptoms and damage degrees under different environmental conditions, the common feature is that bacteria invade from the roots of the plants, and damage the host vascular bundle transport tissue through proliferation and a series of biochemical activities, resulting in dehydration, wilting and death of the plants
So far, it is rare to control bacterial wilt by using crop varieties resistant to bacterial wilt, because the varieties that have emerged are not only low in resistance but also easy to lose resistance. Other agricultural control methods are limited by geographical conditions. Difficult to promote on a large scale
In terms of chemical control, although there are agricultural streptomycin, copper preparations and other drugs, none of them are pesticides for the control of bacterial wilt, and the control effect is poor, unstable, and pathogenic bacteria are prone to develop drug resistance. Effective agent for plant bacterial wilt

Method used

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  • A kind of Paenibacillus macerans for preventing and treating plant bacterial wilt and its application
  • A kind of Paenibacillus macerans for preventing and treating plant bacterial wilt and its application
  • A kind of Paenibacillus macerans for preventing and treating plant bacterial wilt and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Bacterial Strain Isolation and Purification

[0022] The surface soil of 0-20 cm around the cucumber rhizosphere was collected from a cucumber field where bacterial wilt occurred severely in Hangzhou City, Zhejiang Province, put into sterile polyethylene plastic bags, and sealed. Weigh 10g of soil, add it into a triangular flask with 90ml sterile water to prepare a suspension, shake it at 180r / min for 30min, then draw 5ml from the resulting suspension and add it to a triangular flask with 45ml sterile water, and gradually dilute to Until the 5th triangular flask, get 10 -1 、10 -2 、10 -3 、10 -4 and 10 -5 diluent.

[0023] Take 100μl 10 -4 and 10 -5 The diluted solution of smeared on nutrient agar medium (ie NA medium, containing: beef extract 1g, peptone 5g, yeast extract 5g, sodium chloride 5g, sucrose 10g, agar 20g, water 1000ml), each dilution Repeat 3 times, place in a 30°C constant temperature incubator and cultivate for 48 hours, pick a single col...

Embodiment 2

[0030] Example 2 strain identification

[0031] The main morphological and biological characteristics of the strains isolated above are as follows: the cells are rod-shaped, Gram-positive, and move with perinatal flagella. There are oval spores in enlarged cysts, and there is no soluble pigment on nutrient agar. Facultative anaerobic. The colony on the culture medium is thin, expanding in a star shape, with a large amount of bacteria and sticky, and the surface is dull. Growth will adhere to the medium. The optimum temperature for growth is 28-30°C, and the growth temperature range is 15-50°C. The optimum pH for growth is 7.0, and the growth pH range is 5.6-8.5.

[0032] The strain was identified as Paenibacillus macerans by FAME fatty acid analysis and named as Paenibacillus macerans ZJU0902.

Embodiment 3

[0033] Antagonistic activity of embodiment 3 bacterial strain ZJU0902 to Ralstonia solanacearum

[0034] In this example, the 8 R. solanacearum strains in Example 1 were still used as indicator strains. Add 1ml of the bacterial suspension of the indicated strain to 15ml of NA medium that has been sterilized and melted and cooled to about 45°C, shake well and pour the medium into a sterilized petri dish. Pick a single colony of Paenibacillus polymyxa ZJU0902 with a sterilized toothpick, and spot it on the medium with indicator bacteria. The culture condition is 30°C for 48 hours, and its antagonistic ability is determined according to the diameter of the inhibition zone. Antagonism results are as follows (Table 2):

[0035] Table 2 Antagonistic activity of Paenibacillus macerans ZJU0902 against Ralstonia solanacearum strains from different hosts

[0036]

[0037] The above results show that Paenibacillus macerans ZJU0902 has obvious antagonistic activity to other indicator...

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Abstract

The invention discloses a strain of Paenibacillus macerans, which is named as Paenibacillus macerans ZJU0902 after identification, and is preserved in the Chinese Type Culture Collection Center (CCTCC) located at Wuhan University, Luojia Mountain, Wuhan City. The date is July 19, 2009, and the deposit number is CCTCC NO: M 209158. The invention can inhibit the growth of different biochemical strains of R. solanacearum under in vitro conditions, and can effectively prevent and cure plant bacterial wilt.

Description

technical field [0001] The invention relates to a Paenibacillus macerans, in particular to a Paenibacillus macerans for preventing and treating bacterial wilt of plants and an application thereof. Background technique [0002] Bacterial wilt of crops is a systemic and devastating soil-borne disease caused by Ralstonia solanacearum (commonly known as Ralstonia solanacearum), commonly known as "plant blast". The stems and leaves of the affected crops wilt and sag until the whole plant dies. The incidence rate of the general field is 10%-15%, and the incidence rate of the severely diseased field is as high as 80%-99%, resulting in a serious decline in crop yield or even failure. The pathogen has a wide range of hosts and can infect hundreds of plants in 44 families, including tomatoes, eggplants, tobacco, peanuts, potatoes, bananas, etc. [0003] Although there are some differences in the symptoms and damage levels of different plants under different environmental conditions, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01P1/00C12P1/04C12R1/01C12N1/20A01N63/00C07K14/195
Inventor 李斌苏婷余山红张国庆谢关林
Owner ZHEJIANG UNIV
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