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Method for auxiliarily detecting characters of number born of sow and special kit thereof

An auxiliary detection and litter size technology, which is applied in biochemical equipment and methods, microbial measurement/inspection, animal husbandry, etc., can solve problems such as slow progress and low heritability, and achieve accurate judgment, simple operation, and detection Inexpensive effect

Inactive Publication Date: 2012-05-23
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the low heritability (average 0.1) of reproductive traits in sows makes improvement through conventional breeding methods very slow

Method used

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  • Method for auxiliarily detecting characters of number born of sow and special kit thereof
  • Method for auxiliarily detecting characters of number born of sow and special kit thereof
  • Method for auxiliarily detecting characters of number born of sow and special kit thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, establishment of breeding method

[0021] 1. Determination of the R92H polymorphic site

[0022] 1. PCR amplification

[0023] According to the porcine EPOR gene genome DNA sequence (GenBank Accession Number EU407778) information, a pair of primers were designed as follows:

[0024] U (upstream primer): 5'-GCTCTTGGTCAGGGCAATAC-3' (sequence 1 of the sequence listing);

[0025] D (downstream primer): 5'-CCCAGTGCACTTACCCACTT-3' (SEQ ID NO: 2 of the Sequence Listing).

[0026] Beijing black pigs (3 heads) were selected as experimental materials. Using the genomic DNA of Beijing black pig as a template, PCR amplification was performed using primers U and D.

[0027] Amplification system: Genomic DNA 200ng, 10×PCR amplification buffer 2.5μl, dNTPs 5mM, upstream and downstream primers 50ng each, Taq DNA polymerase 0.75U, Mg 2+ 2.5mmol / L, with ddH 2 O Supplement the reaction system to 25 μl.

[0028] The PCR reaction conditions were: denaturation at 95°C fo...

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Abstract

The invention discloses a method for auxiliarily detecting the characters of the number born of a sow and a special kit thereof. The method detects whether the 1645th position of deoxynucleotide of GenBankAccessionNumberEU407778 of the sow to be detected is G or A, and determines whether the genotype of the sow to be detected is GG or AA, and the number born of the sow with GG genotype is higher than that of the sow with AA genotype; the 1645th position of the deoxynucleotide from the 5' tail end with the GG genotype of the GenBankAccessionNumberEU407778 is G homozygote; and the 1645th position of the deoxynucleotide from the 5' tail end with the GG genotype of GenBankAccessionNumberEU407778 is A homozygote. The method can reduce breeding cost, effectively increase the total number born and number born alive of the sow in practical production and improve economical benefit. The method has simple operation and high accuracy, can realize automatic detection and play a great role in breeding of sows.

Description

technical field [0001] The invention relates to a method for assisting in detecting litter size traits of sows and a special kit thereof. Background technique [0002] In modern pig production, the reproductive performance of sows is an important factor affecting the economic benefits of pig farms, so it is also the focus of research on modern pig genetics and breeding. For a long time, the breeding and improvement of pigs worldwide only focused on the selection of growth traits and carcass traits, while the improvement of reproductive traits mostly relied on hybridization. However, the low heritability (average 0.1) of reproductive traits in sows makes improvement through conventional breeding methods very slow. According to Haley, a famous animal population geneticist, as long as the piglet size is increased by 1 head, the British pig industry can obtain an additional profit of 700 million pounds per year, and the additional profit of the entire European Union will reach ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68A01K67/027
Inventor 张龙超王立贤赵克斌颜华
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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