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Method for preparing naringin

A technology of naringin and pore adsorption, applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of low naringin content, unfavorable large-scale production operations, serious pollution, etc.

Inactive Publication Date: 2010-05-12
江苏博益生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the prior art, the extraction and separation methods of naringin mainly use organic solvent methods, etc., but the content of naringin obtained by these extraction processes is low, the pollution in the production process is serious, and it is unfavorable for large-scale production operations

Method used

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  • Method for preparing naringin

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Take 10Kg of Huajuhong coarse powder, add 8 times the volume of water, soak at 30°C for 8 hours, press filter, take the filtrate, add CaCl 2 , stir evenly, place overnight, filter, take the filtrate, add concentrated ammonia water to adjust pH 7-8, absorb through a macroporous adsorption resin column, wash with water to remove impurities, 50% ethanol for elution, and collect 3 times the column volume of the eluate , recover ethanol under reduced pressure and concentrate, refrigerate, precipitate crystals, separate crystals, add absolute ethanol for recrystallization, separate, wash and dry to obtain naringin 88.2g, which is detected by HPLC with a purity of 98.2%.

Embodiment 2

[0019] Take 10Kg of Huajuhong coarse powder, add water 12 times the volume of its weight, soak at 80°C for 24 hours, press filter, take the filtrate, add CaCl 2 , stir evenly, place overnight, filter, take the filtrate, add concentrated ammonia water to adjust the pH7-8, adsorb through a macroporous adsorption resin column, wash with water to remove impurities, 90% ethanol for elution, and collect 8 times the column volume of the eluate , recover ethanol under reduced pressure and concentrate, refrigerate, precipitate crystals, separate crystals, add absolute ethanol for recrystallization, separate, wash and dry to obtain 103.9 g of naringin, which is detected by HPLC with a purity of 98.1%.

Embodiment 3

[0021] Take 10Kg of Huajuhong coarse powder, add water 10 times the volume of its weight, soak at 60°C for 12 hours, press filter, take the filtrate, add CaCl 2 , stir evenly, place overnight, filter, take the filtrate, add concentrated ammonia water to adjust pH 7-8, absorb through D101 macroporous adsorption resin column, wash with water to remove impurities, 75% ethanol for elution, collect 5 times the amount of column volume for elution Liquid, recovered ethanol under reduced pressure and concentrated, refrigerated, precipitated crystals, separated crystals, added absolute ethanol for recrystallization, separated, washed and dried to obtain 118.9 g of naringin, which was detected by HPLC with a purity of 99.2%.

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Abstract

The invention relates to a method for preparing naringin, which is simple in operation, light in pollution and low in equipment investment, and comprises the following process steps of: taking coarse powder of pummelo peel, adding water of which the volume is 8 to 12 times the weight of the coarse powder of pummelo peel into the coarse powder, soaking at the temperature of between 30 and 80 DEG C for 8 to 24h, performing filter pressing, taking filtrate, adding CaCl3 into the filtrate, uniformly stirring the filtrate added with CaCl3, standing the mixture overnight, filtering, taking the filtrate, adding strong ammonia water into the filtrate to adjust the pH to between 7 and 8, adsorbing the filtrate added with strong ammonia water with a macroporous adsorption resin column, eluting with water to remove impurities, eluting with 50 to 90 percent ethanol, collecting the eluate of which the volume is 3 to 8 times that of the column, reclaiming the ethanol at reduced pressure, concentrating the eluate, and refrigerating the eluate to separate out crystal. The naringin prepared by the method is high in purity and easy to realize industrial enlargement.

Description

technical field [0001] The invention relates to a method for preparing naringin, in particular to a method for extracting naringin from plants. Background technique [0002] Naringin (Naringin), synonym: citrusin, naringin, etc., molecular formula: C27H32O14, molecular weight: 580.541, CAS accession number: 10236-47-2, the structural formula is as follows: [0003] [0004] Naringin is a natural compound that has various physiological activities such as anti-inflammatory, anti-tumor, and anti-aging, and is widely found in various plants of Rutaceae. [0005] Huajuhong is the immature or near-ripe dry outer pericarp of Citrus grandis Tomentosa or Citrus grandis (L.) Osbeck of the Rutaceae plant. It has the effects of expelling cold, drying dampness, benefiting qi, and eliminating phlegm. It contains a lot of naringin. [0006] In the prior art, the extraction and separation methods of naringin mainly use organic solvent methods, etc., but the content of naringin obtaine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
Inventor 王琳范淦彬
Owner 江苏博益生物科技有限公司
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