Application of umbilical cord Wharton jelly originated mesenchymal stem cells in heart failure cellular transplantation therapy
A mesenchymal stem cell and cell transplantation technology, applied in the field of mesenchymal stem cells, can solve the problems of reduced para-autocrine function and failure to achieve biological effects, and achieve the effect of improving prognosis and solving high mortality
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0017] Example 1 Preparation of UW-MSCs transplantation materials—Using fresh UW-MSCs
[0018] Fresh umbilical cord Huatong jelly mesenchymal stem cells that have just been subcultured for 2-4 generations were digested with 0.05-0.25% trypsin for 1-5 minutes, serum was neutralized, washed 3 times with balanced salt solution, and the centrifugal force of the centrifuge was controlled. In 100-1000g, it should be resuspended in 0.9% sodium chloride injection containing 0-100 units of heparin / ml for use.
Embodiment 2
[0019] Example 2 Preparation of UW-MSCs transplantation material - application of cryopreserved UW-MSCs spares
[0020] Take out the frozen 2-4 generation umbilical cord Huatong jelly mesenchymal stem cells from the cryogenic container, quickly put them into a 35-38°C incubator and water bath, dissolve within 2 minutes, and resuspend the cells with complete medium solution. After 2-4 days of cell fusion, re-digest and wash the cells, use heparin sodium chloride injection with a concentration of "1000 units of heparin: 100 ml of 0.9% sodium chloride solution" to make a cell concentration of 1-2×10 6 / ml of cell suspension, usually packed in 10ml tubes.
Embodiment 3
[0021] Example 3 Surgical method of transvenous transplantation of UW-MSCs
[0022] Will 2×10 7 The UW-MSCs suspension was diluted in 20ml heparin saline (heparin 200 units + normal saline 20ml) and injected rapidly from the vein.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com