Biomimetic tissue culture room

Abstract

The invention discloses a biomimetic tissue culture room, a lower part container and an upper cover of which are made of medical plastic; wherein the upper cover is molded with a stretch folding side in an integrated way, and the stretch folding side of the upper cover is connected with the lower part container to form a sealed culture room; the lower part container is internally provided with a vertical upward bracket for supporting biological tissue; the upper cover is internally provided with a device which extends downwards and is used for applying stress; the bracket for supporting the biological tissue and the device used for applying stress are positioned on the same axle center line, and a space used for placing the biological tissue is arranged between the bracket for supporting the biological tissue and the device used for applying stress; the upper end of the upper cover is provided with a connecting rod connected with a power transmission mechanism; and the lower part container is respectively provided with a culture solution input hole and a culture solution output hole. According to the properties of different culture tissues, the biomimetic tissue culture room leadsthe culture tissue to be raised in a way of simulating blood circulation and applied with biological stress stimulation imitating different organism physiological states.

Description

technical field [0001] The invention relates to a biological tissue engineering preparation device, in particular to a bionic tissue culture room prepared by biological tissue engineering. Background technique [0002] The in vitro biomimetic culture of biological tissues refers to the cultivation of tissue engineered tissues constructed by applying tissue engineering technology under in vitro culture conditions that simulate the physiological environment in vivo, so as to promote the structural and functional maturation of tissue engineered tissues. This kind of bionic cultivation must be realized by means of the application of the cultivation chamber. The biomimetic simulation of the physiological environment particularly needs to involve two aspects of biomimicry, that is, the simulation of blood circulation in the way of supplying the culture medium, and the simulation of the physiological stress environment and mode in the stress application. The more advanced tissue c...

AI Technical Summary

Problems solved by technology

Such a structure will lead to the air flow driven by the opening of the culture container and the movement of the connecting rod during the culture process, making the tissue culture process easily contaminated by bacteria and failing, making it difficult to complete long-term, high-quality tissue culture and experimental observation

Moreover, the compressive stress applying culture room adopts this structure, and its compressive stress applying device is mainly electromagnetic control, which can only control the frequency and displacement of compressive stress, but cannot control the magnitude and action form of the applied compressive stress. It is an impact rectangular wave, and the biological compressive stress is mostly a parabolic wave. Therefore, the compressive stress obtained by the existing tissue culture room cannot imitate the biological stress microenvironment in the organism well, and cannot be well Realizing the biomimetic application of compressive stress has limited effectiveness in promoting tissue engineering tissue structure and functional maturation, and even has the opposite effect, and the structure of the culture container determines that only a single compressive stress stimulus can be applied.

At the same time, the existing stress-applied culture chamber is an open culture container with simple structure and single function, which leads to manual operation for the renewal of culture medium, which is easy to artificially cause pollution in the culture process, and the stability of the cultured microenvironment is poor. detrimental to tissue development

Because the culture container with this structure places the culture tissue directly on the bottom of the container, the nutrient exchange surface of the culture tissue is reduced, which affects the support exchange of the tissue.

[0005] In particular, the existing two types of tissue culture rooms are generally reused after disinfection because the materials for making culture containers are more expensive, and repeated use requires strict cleaning and disinfection. Once the disinfection is not strict, it will cause cross-infection

[0006] Therefore, the existing bionic tissue culture room is generally only suitable for laboratory research on the effect of a single factor on the in vitro generation of tissue engineered tissue due to its single function, and cannot simulate the in vivo environment, so the promotion effect on the in vitro development of tissue engineered tissue is limited and cannot Adapting to the requirements of tissue engineering tissue biomimetic cultivation research and the needs of clinical applications, improving the design of special tissue culture chambers and their support systems for various purposes has become a hot spot in tissue engineering technology research in recent years

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