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Effective part and active ingredient of madder for lowering blood sugar, its preparation method and application

A technology of effective parts and active ingredients, which is applied to the effective part of madder for lowering blood sugar and its preparation field, can solve the problems of high cost and intestinal side effects, and achieve the effect of safe price, good social value and economic value, and rich sources

Inactive Publication Date: 2012-02-01
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing drugs of this type are costly and have intestinal side effects

Method used

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  • Effective part and active ingredient of madder for lowering blood sugar, its preparation method and application
  • Effective part and active ingredient of madder for lowering blood sugar, its preparation method and application
  • Effective part and active ingredient of madder for lowering blood sugar, its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] This example is the extraction method and preliminary activity screening of each extract of Rubia radiata, that is, the effective part.

[0023] The preparation method of the effective part of madder for lowering blood sugar is as follows: crush 500 g of madder rhizome, moisten with acetic acid, extract twice with chloroform and methanol respectively at room temperature, filter, recover the solvent, and obtain the effective part of chloroform for lowering blood sugar (Rc, 4.5g) and methanol hypoglycemic effective fraction (Rm, 12.8g).

[0024] Activity assay:

[0025] Method: Microplate method

[0026] Principle: α-D-glucosidase catalyzes the hydrolysis of 4-nitrobenzene-α-D-glucopyranoside (PNP-G) to produce nitrophenol (PNP, a yellow substance with maximum absorption at around 400m), α -Glycosidase inhibitors can inhibit the binding of α-glucosidase to the substrate to reduce the release of PNP. The enzyme inhibitory activity of the extract was calculated by the ch...

Embodiment 2

[0036] This example is a method for preparing the active ingredient, that is, the active compound, from the active part of Rubia chloroform under the guidance of activity.

[0037] The specific method is: the effective part of chloroform (4.5g) obtained in Example 1 is subjected to 200-300 mesh silica gel column chromatography, petroleum ether-acetone gradient elution (95:5-9:1), and 4 parts are obtained, of which Fraction 3 (Rc-3, 1.6 g) had α-glucosidase inhibitory activity (Table-2). This part was eluted by silica gel H column chromatography, petroleum ether-acetone (15:1), combined with TLC detection, and subjected to Sephadex LH-20 column chromatography, acetone eluted to obtain compound 1 (12.5mg), compound 2 (5.5mg ) and compound 3 (120.7 mg).

Embodiment 3

[0039] This example is the determination of the activity of the compounds in Rubia radiata inhibiting α-glucosidase.

[0040] Measuring method: with embodiment 1.

[0041] Determination results: The experimental results are shown in Table 2. The initial screening inhibition rates of the three compounds were all higher than the positive control Acarbose, while the IC 50 Values ​​are all lower than 50μg·mL -1 , far less than the clinically commonly used hypoglycemic drug Acarbose (IC 50 =1081.27μg·mL -1 ), has high α-glucosidase inhibitory activity, and can be developed as an active ingredient of madder for α-glucosidase inhibition.

[0042] The α-glucosidase inhibitory activity of active ingredient in table 2 madder

[0043]

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Abstract

The present invention relates to an effective part of madder for lowering blood sugar and a preparation method thereof, and also relates to an active ingredient extracted from the effective part for lowering blood sugar and a preparation method thereof, and also relates to the application of madder in reducing blood sugar and its effective part and its preparation method. Application of active ingredients. The effective part is the effective part of chloroform and the effective part of methanol which are obtained by crushing and wetting the roots of the medicinal plant madder, and successively leaching with chloroform and methanol. The present invention uses the traditional Chinese medicine madder as raw material, which is rich in sources and has the characteristics of relative safety and low price as a natural herbal medicine. value. In addition, it plays an important role in the development of efficient and safe new drugs for reducing postprandial blood sugar.

Description

technical field [0001] The present invention relates to an effective part of madder for lowering blood sugar and a preparation method thereof, and also relates to an active ingredient extracted from the effective part for lowering blood sugar and a preparation method thereof, and also relates to the application of madder in reducing blood sugar and its effective part and its preparation method. Application of active ingredients. Background technique [0002] Diabetes mellitus is a systemic disease characterized by hyperglycemia, metabolic disorders, and closely related to insulin secretion. It is a clinical syndrome caused by the interaction of genetic and environmental factors. Absolute or relative insufficiency of insulin secretion and decreased sensitivity of target tissues to insulin lead to a series of metabolic disorders of sugar, protein, fat, water and electrolytes. Diabetes is mainly divided into two types: type I diabetes (ie, insulin-dependent diabetes) and type ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/74A61P3/10C07C50/18
Inventor 康文艺李昌勤王艳鸽张丽宋艳丽
Owner HENAN UNIVERSITY
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