A germinated brown rice rich in bioactive peptides
A bioactive peptide and germinated brown rice technology, applied in the field of food biology, can solve problems such as difficult consumer acceptance and poor taste of brown rice, and achieve the effects of easy operation, easy industrial production, and scientific technology
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Embodiment 1
[0047] The paddy is removed from the husk by a rice paddy machine to obtain brown rice. Select brown rice with full grains, immerse in 1% sodium hypochlorite solution for disinfection for 30 minutes, and then rinse with clean water several times. Soak the sterilized brown rice in water for 6 hours, change the water once for 3 hours, 15 minutes each time, put the brown rice in a nylon mesh bag, put it into the culture medium according to the ratio of brown rice: culture medium 1:5, soak and ventilate, and cultivate The liquid composition is gibberellin 55μmol / L, NaCl 6.0mmol / L and MnSO 4 1.8mmol / L, the ventilation volume is 2.0L·min -1 , germinate under dark culture conditions at 32°C, replace the culture medium every 12h, take out the brown rice after germinating for 60h; scald for 6min at a water temperature of 90°C, with a solid-liquid ratio of 1:1, and cool down after inactivating the enzyme; at 40°C~ Drying at 65°C, the finished germinated brown rice is light yellow, sh...
Embodiment 2
[0049] Brown rice preparation and selection, disinfection, soaking and other treatments are the same as in Example 1. According to the ratio of brown rice: culture solution 1:5, put it into the culture solution to soak and ventilate. The composition of the culture solution is gibberellin 30μmol / L, NaCl 2.0mmol / L and MnSO 4 0.6mmol / L, the ventilation volume is 0.1L·min -1 , culture temperature 16 ℃, germination 36h, every 12h culture medium is replaced once, and post-germination treatment is the same as embodiment 1. After drying, the germinated brown rice finished product is light yellow, shiny, uniform in body shape, and the content of bioactive peptide is 11.49mg g -1 .
Embodiment 3
[0051] Brown rice preparation and selection, disinfection, soaking and other treatments are the same as in Example 1. According to the ratio of brown rice:culture solution 1:5, put it into the culture medium to soak and ventilate. The culture medium consists of gibberellin 80μmol / L, NaCl 12mmol / L and MnSO 4 3.0mmol / L, ventilation volume 3.0L min -1 , culture temperature 26 ℃, cultivate 40h, change water once every 12h, post-germination treatment is the same as embodiment 1. After drying, the germinated brown rice finished product is light yellow, shiny, and uniform in shape, and the content of bioactive peptide is 12.13mg g -1 .
[0052] Table 1 Plackett-Burman experimental design and measurement data
[0053]
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