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Preparation method of inactivated vaccine of infectious coryza of chicken

A technology for chicken infectious rhinitis and inactivated vaccine, which is applied in the field of inactivated vaccine preparation, can solve the problems of complex process, high production cost, low yield and the like, and achieves the advantages of increasing the number of cultured bacteria, simple and feasible operation, and reducing production cost. Effect

Active Publication Date: 2012-05-02
哈药集团生物疫苗有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is to overcome defects such as complex process, high production cost and low yield in the existing preparation method of chicken infectious rhinitis inactivated vaccine, and provide a new inactivated chicken infectious rhinitis vaccine. A preparation method, the preparation method has the advantages of simple and convenient process, low production cost and high yield;

Method used

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  • Preparation method of inactivated vaccine of infectious coryza of chicken

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Preparation of primary slant strains: dilute Haemophilus paragallinarum strains and inoculate chicken agar plates at 37°C for 22 hours, select 10 typical colonies to inoculate chicken agar slant medium, and cultivate at 37°C for 24 hours to obtain primary slant strains. Place in a 4°C refrigerator for later use;

[0022] 2. Preparation of secondary strains: Inoculate the primary slant strains into the chicken broth culture medium and incubate at 37°C for 12 hours in a full-temperature shaker. After the pure test confirms the sterility, obtain the secondary strains for future use;

[0023] 3. Bacterial liquid culture: inoculate the secondary strains into semi-synthetic medium and cultivate them in a biological fermentation tank; culture temperature: 37°C, rotation speed: 100r / min, culture time 8h; count live bacteria on the sampling plate and confirm that there is no After sterilizing, it is inactivated, and it is obtained.

[0024] Using the "Regulations for Veterin...

Embodiment 2

[0026] 1. Preparation of primary slant strains: dilute Haemophilus paragallinarum strains and inoculate chicken agar plates at 37°C for 24 hours, select 20 typical colonies to inoculate chicken agar slant medium, and cultivate at 37°C for 24 hours to obtain primary slant strains. Place in a 4°C refrigerator for later use;

[0027] 2. Preparation of secondary strains: Inoculate the primary slant strains into the chicken broth culture medium and incubate at 37°C for 12 hours in a full-temperature shaker. After the pure test confirms the sterility, obtain the secondary strains for future use;

[0028] 3. Bacterial liquid culture: Inoculate the secondary strains into semi-synthetic medium for cultivation in a biological fermentation tank; cultivation temperature: 37°C, rotational speed: 200r / min, cultivation time 8h; The method of large ventilation volume; after taking a sample plate and counting the live bacteria to confirm the sterility, it is inactivated to obtain the product. ...

Embodiment 3

[0031] 1. Preparation of primary slant strains: dilute Haemophilus paragallinarum strains and inoculate chicken agar plates at 37°C for 23 hours, select 15 typical colonies to inoculate chicken agar slant medium, and cultivate at 37°C for 24 hours to obtain primary slant strains. Place in a 4°C refrigerator for later use;

[0032] 2. Preparation of secondary strains: Inoculate the primary slant strains into the chicken broth culture medium and incubate at 37°C for 12 hours in a full-temperature shaker. After the pure test confirms the sterility, obtain the secondary strains for future use;

[0033] 3. Bacterial liquid culture: inoculate the secondary strains into semi-synthetic medium and cultivate them in a biological fermentation tank; culture temperature: 37°C, rotation speed: 150r / min, culture time 8h; count live bacteria on the sampling plate and confirm that there is no After sterilizing, it is inactivated, and it is obtained.

[0034] Using the "Regulations for Veterin...

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Abstract

The invention discloses a preparation method of an inactivated vaccine of infectious coryza of chicken, which comprises the following steps: firstly, diluting haemophilus paragallinarum strains, inoculating a chicken agar plate, culturing at 37 DEG C for 22-24h; selecting a typical colony to inoculate a chicken agar slant culture-medium, culturing to obtain first class slant strains; secondly, getting the first class slant strains to inoculate into a chicken soup culture medium, vibrating and culturing at 37 DEG C for 12h, obtaining second class strains; thirdly, inoculating the second class strains into a semisynthetic culture medium, culturing by a biological fermentation cylinder; and inactivating to obtain the inactivated vaccine. Aiming at defects of complex process, high production cost, low yield and the like existing in the prior preparation method of the inactivated vaccine of infectious coryza of chicken, the research work of an improved production process is developed, and the important breakout on the culture method is obtained. The method has simple and feasible operation; compared with the prior art, the invention greatly improves the culture strains, shortens the culture time by 10-12h, and effectively lowers the production cost.

Description

technical field [0001] The invention relates to a method for preparing an inactivated vaccine, in particular to a method for preparing an inactivated vaccine for chicken infectious rhinitis, and belongs to the field of inactivated vaccine preparation. Background technique [0002] Chicken infectious rhinitis is an acute respiratory infectious disease caused by Haemophilus paragallinarum. The main feature is inflammation of the eye and nasal mucosa in varying degrees. Significant declines occur most often in 8-12 week old chickens and laying hens. The disease is distributed all over the world. Due to the 10%-40% reduction in egg production of infected laying hens, stagnant weight gain of growing chickens and increase in the number of culled chickens, it often causes serious economic losses. Although the disease can be treated with antibiotics and sulfonamides, it will cause drug residues. According to field surveys, more than 90% of large-scale chicken farms use vaccination ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/02A61P31/04A61P11/02C12N1/20C12R1/01
Inventor 吴金张继东张明波付丽杰尹尧王华刘方悦
Owner 哈药集团生物疫苗有限公司
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