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Modified flagellin improved toll-like receptor 5 stimulating activity

A technology of flagellin and receptors, which is applied in the direction of antibody medical ingredients, medical preparations containing active ingredients, decapeptides, etc., can solve the problem of lack of vaccine adjuvants in vaccines, and achieve high value-added effects

Active Publication Date: 2010-01-06
李浚行 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] A common problem observed in prophylactic and therapeutic vaccines (vaccines for infectious diseases, autoimmune diseases, allergic diseases, and cancer) currently used in clinical practice is that these vaccines lack effective vaccines that can specifically amplify the relevant response. Vaccine Adjuvant

Method used

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  • Modified flagellin improved toll-like receptor 5 stimulating activity
  • Modified flagellin improved toll-like receptor 5 stimulating activity
  • Modified flagellin improved toll-like receptor 5 stimulating activity

Examples

Experimental program
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Embodiment 1

[0039] Example 1: Preparation of recombinant flagellin mutants by site-directed mutagenesis

[0040] According to the analysis data of the three-dimensional structure of Salmonella flagellin previously reported (Yonekura K, Maki-Yonekura S, Namba K: Complete atomic model of the bacterial flagellarfilament by electron cryomicroscopy. Nature. 2003424(6949): 643-50; SamateyFA, Imada K , Nagashima S, Vonderviszt F, Kumasaka T, Yamamoto M, Namba K: Structure of the bacterial flagellar protofilament and implications for a switch for supercoiling. Nature. 2001410(6826): 331-7), and bioinformatics conducted by the inventors Analyzing, the amino acid residues required for axial interaction between V. vulnificus flagellin FlaB monomers are as follows. That is, the aspartic acid (D70) at the 70th position of the upper subunit (upper subunit), the asparagine (D135) at the 135th position of the upper subunit, and the upper subunit of each monomer participating in the axial interaction The...

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Abstract

Disclosed herein are flagellin mutants having an enhanced activity of stimulating the toll-like receptor-5 (hereinafter referred to as ''TLR5''). More specifically, disclosed are flagellin mutants, prepared by point-mutating some of the amino acids of a TRL5 agonist flagellin so as to enhance the TRL-stimulating activity of the flagellin.

Description

technical field [0001] The present invention relates to flagellin mutants with enhanced toll-like receptor-5 (hereinafter referred to as "TLR5") stimulating activity, in particular to enhancing the TLR5 stimulating activity of flagellin by point-mutating certain amino acids of the TRL5 agonist flagellin The flagellin mutants were prepared. Background technique [0002] Flagella is an important structural element that determines bacterial motility, and it usually consists of a hook, a matrix, and a filament. Flagella are known to contribute to swimming or swarming movements, bacterial tropism, adhesion of pathogenic microorganisms to host cells, and biofilm formation. The subunit proteins that form the flagellin filaments are called flagellins, and the flagellins assemble regularly to form filaments. Hayashi et al. disclosed that TLR5 expressed in mammalian cells can recognize flagellin of Gram-negative and Gram-positive bacteria to activate NF-κB (Hayashi F, Smith KD, Ozin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/255
CPCA61K39/107A61K39/39C07K14/28
Inventor 李浚行李施恩金守永
Owner 李浚行
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