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Early detection kit of nasopharyngeal carcinoma-related gene EBNA1 and detection method and application thereof

A detection kit and early detection technology, applied in the direction of microbial determination/inspection, biochemical equipment and methods, etc., to achieve stable reagents, broad market prospects, and good repeatability

Inactive Publication Date: 2009-12-09
SUN YAT SEN UNIV
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At present, there is no detection of EBNA1 gen

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  • Early detection kit of nasopharyngeal carcinoma-related gene EBNA1 and detection method and application thereof

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Abstract

The invention provides an early detection kit of nasopharyngeal carcinoma-related gene EBNA1, comprising gene specific primers, dNTP, DNA polymerase and buffering liquid of the DNA polymerase; wherein the gene specific primers are used for amplifying the nasopharyngeal carcinoma-related gene EBNA1, and the DNA polymerase is used for PCR reaction. The invention also provides a method for the early detection of the nasopharyngeal carcinoma-related gene EBNA1 and applications thereof. The detection kit and the detection method of the invention have the advantages of simple operation, stable agent, good repeatability, high specificity and sensitivity, and batch PCR detection, are valuable indexes for screening nasopharyngeal carcinoma in large scale, have wide market prospects and have significant clinical value in screening of the high risk group of nasopharyngeal carcinoma and early detection and diagnosis of nasopharyngeal carcinoma.

Description

technical field [0001] The invention relates to a nasopharyngeal carcinoma early detection kit and a detection method, in particular to a nasopharyngeal carcinoma related gene EBNA1 early detection kit and its detection method and application. Background technique [0002] Nasopharyngeal carcinoma (NPC) is a common malignant tumor in southern China and Southeast Asia, with an incidence rate of 15-50 / 100,000 population. A variety of genetic and environmental factors are involved in the occurrence of nasopharyngeal carcinoma, among which Epstein-Barr virus (Epstein-Barr virus), as a special environmental pathogenic factor, is highly related to nasopharyngeal carcinoma: Epstein-Barr virus in the serum of patients with nasopharyngeal carcinoma is specific Epstein-Barr virus infection occurred before the monoclonal proliferation of nasopharyngeal carcinoma cells; Epstein-Barr virus DNA and its expression products were widely detected in nasopharyngeal carcinoma lesions, all of wh...

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 买世娟谢丹孔祥复黄宇帆廖奕佶邓海霞
Owner SUN YAT SEN UNIV
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