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Arbuscular mycorrhizal fungus for repairing atrazine-contaminated soil

A technology of arbuscular mycorrhizal fungi and atrazine, which is applied in the field of microorganisms and can solve the problems of unsatisfactory effects of atrazine-contaminated soil

Inactive Publication Date: 2011-04-06
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problem that the current bioremediation of atrazine-contaminated soil has an unsatisfactory effect, and provides an arbuscular mycorrhizal fungus for repairing atrazine-contaminated soil

Method used

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  • Arbuscular mycorrhizal fungus for repairing atrazine-contaminated soil
  • Arbuscular mycorrhizal fungus for repairing atrazine-contaminated soil
  • Arbuscular mycorrhizal fungus for repairing atrazine-contaminated soil

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specific Embodiment approach 1

[0013] Specific embodiment one: the arbuscular mycorrhizal fungi repairing atrazine-contaminated soil in this embodiment is Glomus mosseae (GM) HDSF1, which is preserved in the General Microorganism Center (CGMCC) of China Microbiological Culture Collection Management Committee, The deposit number is CGMCC No.3012, and the deposit date is April 09, 2009.

[0014] In this embodiment, the arbuscular mycorrhizal fungus Glomusmosseae (GM) HDSF1 used to remediate atrazine-contaminated soil was collected from farmland soil polluted by pesticide atrazine residues in Yangmajiazi, Harbin.

[0015] Glomus mosseae (GM) HDSF1 was collected and purified according to the following steps: 1. The soil polluted by the pesticide atrazine residue was excavated at a depth of 0-30 cm, and then the arbuscular branches were separated and purified from the soil by wet sieving. Mycorrhizal fungal spores; 2. Soak the arbuscular mycorrhizal fungal spores in a mixed solution in which the mass concentrati...

specific Embodiment approach 2

[0016] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the spores of Glomus mosseae (Glomus mosseae, GM) HDSF1 are yellow or brown, spherical, and the average diameter is 210 μm; mosseae, GM) HDSF1 conidia hyphae have a funnel-shaped base with a diameter of 20-30 μm; mature Glomus mosseae (GM) HDSF1 spore wall divides from outside to inside. 2 and L 3 two floors, L 2 The layer is light yellow, with a thickness of 1.2-1.6 μm, L 3 layer with L 2 The layers were separated, yellowish brown in color, and the average thickness was 4.5 μm. Others are the same as the first embodiment.

[0017] The L1 layer of mature Glomus mosseae (GM) HDSF1 spores has been shed.

[0018] mature Glomus mosei HDSF1 spores such as Figure 1~6 Shown (mature spores of 6 different G. mosei HDSF1).

[0019] The hyphae of Glomus mosseae (GM) HDSF1 have a curved thick septum that separates the spore contents from those of the hyphae. Through observation,...

specific Embodiment approach 3

[0020] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: immature Glomus mosseae (Glomus mosseae, GM) HDSF1 spore wall divides from outside to inside. 1 , L 2 and L 3 3rd floor, L 1 The layer is located on the surface of the spores and is transparent, stained pink by Melzer's reagent, with an average thickness of 3.2 μm. Others are the same as the first or second embodiment.

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Abstract

The invention provides an arbuscular mycorrhizal fungus for repairing atrazine-contaminated soil and relates to a microorganism, in particular to an arbuscular mycorrhizal fungus, solving the problem that the effect of the existing method adopting organisms to repair the atrazine-contaminated soil is unsatisfactory. Moses sacculus mildew HDSF1 is preserved in the Common Microorganism Center of China Committee for Culture Collection of Microorganisms, with the preservation number of CGMCC No. 3012. After the Moses sacculus mildew HDSF1 is inoculated in the soil contaminated by atrazine, the growing trend of a crop plant sown on the soil is good, and the biomass is obviously higher than that not inoculated with the repairing arbuscular mycorrhizal fungus of the invention; and the residual quantity of the atrazine in the soil is greatly reduced. In the arbuscular mycorrhizal fungus for repairing the atrazine-contaminated soil, the Moses sacculus mildew HDSF1 is not influenced by indigenous microorganism contamination in the repaired soil, and the anti-interference property is strong; and under the condition that the concentration of the atrazine in the soil is lower, favorable biological degradability can still be kept, and the biological activity is kept.

Description

technical field [0001] The invention relates to a microorganism, in particular to an arbuscular mycorrhizal fungus. Background technique [0002] Atrazine (2-chloro-4-ethylamino-6-isopropylamino-1) is mainly used as an agricultural herbicide. Due to long-term use, a large amount of residue remains in the soil, and the yield of crops is greatly reduced. The remediation of atrazine-contaminated soil can use physical, chemical and biological methods, because the physical treatment method only transfers the pollutants, cannot completely eliminate atrazine, and is expensive; while the chemical treatment method is likely to cause the contamination of other environmental media. Secondary pollution; therefore, biological methods are mainly used to deal with atrazine. [0003] Bioremediation of atrazine-contaminated soil is divided into two categories: the first method is to use microbial strains with atrazine-degrading ability to remediate atrazine-contaminated soil, but because th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14B09C1/10C12R1/645
Inventor 宋福强孟剑侠丁明玲范晓旭
Owner HEILONGJIANG UNIV
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