Method for establishing hybrid rice dual-purpose maintainer line

Abstract

The invention relates to a novel breeding method for establishing a hybrid rice dual-purpose maintainer line. A photo-sensitive rice male sterile line used as a female parent is hybridized with a cytoplasmic male sterility maintainer line; and after replacement and self-crossing selective separation, not only a photo-sensitive recessive nucleus male sterile gene but also a cytoplasmic male sterility maintainer gene are established. On the conditions of long day and high temperature, the sterility is controlled by the photo-sensitive male sterile gene; on the conditions of short day and low temperature, the sterility is controlled by the cytoplasmic male sterility gene, thus solving the problems that a cytoplasmic male sterility line is affected by high temperature and fertility and abortion are not thorough. The invention integrates the cytoplasmic male sterility maintainer gene with the photo-sensitive male sterile gene leading to one line in two uses, and the risk that due to abnormal temperature fertility and abortion are not thorough in the process of production of hybrid rice parent sterility maintainer line can be avoided, thus ensuring the safety for producing hybrid rice seeds and multiplying parental seeds.

Description

technical field [0001] The invention relates to a breeding method for hybrid rice parents, in particular to a new method for creating dual-purpose male sterile maintainer lines. Background technique [0002] The three-line hybrid rice has made a great contribution to greatly increasing the yield per unit of rice. The two-line hybrid rice is not restricted by the restoration relationship, and can be freely formulated and combined, which greatly expands the range of genetic resources available for rice. Excellent hybrid rice combinations provide a wide range of basic conditions. Whether it is two-line or three-line hybrid rice, the fertility stability of the sterile line is very important. The essence of the sterile line is the homokaryotic heterogeneity of the maintainer line. Most of the genetic factors of the plant exist in the nucleus. The reproductive stability and recoverability are related to the genotype of the maintainer line. In the actual production of three-line...

AI Technical Summary

Problems solved by technology

However, the cytoplasmic male sterility of these sterile lines is greatly affected by temperature. Under normal temperature conditions, the cytoplasmic male sterility gene is normally expressed, and the pollen is completely sterile. The fertility of the sterile line fluctuates with the change of the environment and climate

Changes in weather, high and low temperatures, and uncontrollable stability have become the most critical issues. Therefore, there are risks in the production of hybrid rice seeds