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Seed production method of nk971 three-line matching hybrid

A technology of hybrid seed production and inbred lines, applied in the biological field, can solve the problems of long transfer cycle

Active Publication Date: 2016-08-24
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, only using the traditional backcrossing transfer method usually requires backcrossing for 5-6 generations, and the transfer cycle is longer, which delays the application of sterile seed production technology in corn hybrids

Method used

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  • Seed production method of nk971 three-line matching hybrid
  • Seed production method of nk971 three-line matching hybrid
  • Seed production method of nk971 three-line matching hybrid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Embodiment 1, the breeding of male sterile line S Jing 724

[0085] 1. Determination of the type of male sterile line donor MD32 cytoplasmic male sterility

[0086] 1. Maize male sterile line MD32

[0087] Breeding process of maize male sterile line MD32: use the constructed X line population (based on X1132) to carry out "high and strict" line selection, and select excellent S5 high-generation lines and introduced hybrids to construct a new basic line selection population. Sterile plants were found in the selfed progeny, and the anthers of the tassels were not exposed, and sister plants with similar phenotypes in the same panicle row were selected for pair pollination. The hybrid offspring all showed complete sterility, and the plant phenotype tended to be basically the same. Then the material was named maize male sterile line MD32.

[0088] The corn sterile line MD32 is completely sterile: the anthers are not exposed;

[0089] The sterility of maize sterile line M...

Embodiment 2

[0133] Embodiment 2, the transfer of female parent Jing 388 male sterile line S Jing 388

[0134] S Jing 724 was used as the female parent, and Jing 388 (Corn Research Center of Beijing Academy of Agriculture and Forestry Sciences, the variety right application announcement number is CNA010624E) was used as the male parent. 1 Generation, F 1 The generation field showed complete sterility;

[0135] then F 1 Make the female parent, and continue to backcross with Jing 388 to get BC 1 generation groups.

[0136] From the planted 2000 BC 1 620 individual plant listing markers with complete male flower sterility and plant traits (plant height, ear height, axis color, number of tassel branches, ear traits, growth period, etc.) similar to those of Jing 388 were selected from the generation population; BC 1 The DNA of a single plant leaf of the first generation was used as a template, and 23 pairs of primers (bnlg439w1, umc1335y5, bnlg1940k7, umc2105k3, umc1705w1, bnlg1702k1, umc154...

Embodiment 3

[0143] Embodiment 3, the restorative identification of Jing 372 to the sterile line S Jing 388

[0144] With the male sterile line S Jing 388 created by Example 2 as the female parent, Jing 372 (recorded in the following documents: "Seed World" 2014 4th issue summer maize NK971 basic characteristics and high-yield cultivation techniques, the public can obtain from Beijing Agriculture and Forestry Acquired by the Academy of Sciences) as the male parent, hybrid combination F 1 Generation, Harvest F 1 generation of seeds. The next year, plant F in Beijing, Hainan, Jilin and other fields 1 Generation, get F 1 Substitute plants. Observation identification F 1 Fertility restoration of tassels in generation plants showed that fertility returned to normal.

[0145] It shows that Jing 372 is a strong restorer line of the male sterile line S Jing 388 and has full recovery ability.

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Abstract

The invention discloses a NK971 three-line matching hybrid seed production method. The method comprises that a maize sterile line S Jing 388 as a sterile line, a maize inbred line Jing 388 as a maintainer line and a maize inbred line Jing 372 as a restorer line are subjected to three-line matching seed production so that hybrid seeds NK971 are obtained. An experiment proves that the method utilizes the bred sterile line S Jing 388, maintainer line Jing 388 and restorer line Jing 372 to realize three-line matching hybrid seed production and can produce the sterile hybrid seeds NK971 with yield, resistance and other agronomic characters the basically same as those of seeds NK971 produced by the conventional seed production method.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for producing NK971 three-line hybrid hybrids. Background technique [0002] Breeding and promotion of strong dominant hybrids is an important way to increase maize yield. The use of conventional seed production methods to prepare hybrids requires manual detasseling of the female parent, which not only consumes a lot of labor, increases the cost of seed production, but also has the potential risk of affecting seed quality due to untimely and incomplete detasseling. The use of male sterile lines for seed production is an effective method to ensure seed purity and increase maize yield. [0003] As early as the 1950s and 1960s, research on maize sterile seed production began at home and abroad. Cytoplasmic male sterility is the main type used in maize breeding because it is easy to realize the matching of sterile lines, maintainer lines and restorer lines. Cytoplasmic male s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
Inventor 赵久然宋伟邢锦丰王元东段民孝刘春阁冯培煜张如养王凤格毛振武李瑞媛王乃顺王文广张莎莎
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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